[Effects of Nanomembrane Covering and Bacillus Inoculant Application on Bacterial Community Structure, Key Clusters, and Substance Transformation Process in Aerobic Manure Composting System of Livestock and Poultry].

Bacillus, which has good cellulase secretion function, heat resistance, and good environmental adaptability, plays a key role in aerobic composting. In the aerobic composting process, nanomembrane coating can provide a suitable microclimate； however, the role of it in promoting the function of Bacillus inoculants in the aerobic composting system remains clear. In this study, the fermentation experiment of tank aerobic compost was set up, and four aerobic compost treatment groups were established： control group（CK）, single inoculation of Bacillus（CB）, nanomembrane covering（CM）, and nanomembrane covering combined with Bacillus inoculation（BM）. The effects of nanomembrane covering and Bacillus inoculation on aerobic compost were discussed. The effects of microbial community structure on microbial metabolic activity in aerobic compost were analyzed using redundancy analysis and the bacterial ecological network. The results showed that the BM treatment could promote the degradation of organic matter and increase the temperature of the reactor. After adding Bacillus, the enzyme activity of the CM treatment changed more than that of the CK treatment. The relative abundance of Firmicutes and Actinobacteria, which are related to organic matter metabolism, is more abundant when covered by the BM treatment. Simultaneously, the relative abundance of core bacterial cluster 3 showed a significant positive correlation with the material conversion ability of aerobic compost, and the relative abundance of bacterial cluster 3 in the BM treatment group was observed to be higher than that in the other treatment groups.