Lauren Kline Lovejoy, Joe P. Foley, Kaitlin M. Grinias
{"title":"串联柱液相色谱连续洗脱对弱酸、中性化合物和永久离子的高效液相色谱分离验证。","authors":"Lauren Kline Lovejoy, Joe P. Foley, Kaitlin M. Grinias","doi":"10.1002/jssc.70124","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Sequential elution-liquid chromatography (SE-LC) employs conventional high-performance LC instrumentation to separate compounds by class and within each class using two or more sequential elution modes. The aim of this work was to validate a novel SE-LC method used to separate weak acids, permanently charged ions (anions), and neutral compounds by applying first a low pH isocratic elution to elute the weak acids, then an acetonitrile gradient to elute the neutral compounds, and lastly a sodium methanesulfonate gradient to elute the anionic compounds using a tandem-column arrangement comprised of a C18 column and a strong anion exchange column. The effects of flow rate, starting mobile phase organic content, column temperature, mobile phase composition, and gradient start time were investigated using a 2<sup>5-1</sup> fractional factorial design with center points for robustness. The method was demonstrated to be robust, specific, linear, accurate, and precise (repeatable) over the range of 6%–120% of the target analyte concentrations.</p>\n </div>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 3","pages":""},"PeriodicalIF":2.8000,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Validation of a High-Performance Liquid Chromatography Separation of Weak Acids, Neutral Compounds, and Permanent Ions Using Sequential Elution with Tandem-Column Liquid Chromatography\",\"authors\":\"Lauren Kline Lovejoy, Joe P. Foley, Kaitlin M. Grinias\",\"doi\":\"10.1002/jssc.70124\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>Sequential elution-liquid chromatography (SE-LC) employs conventional high-performance LC instrumentation to separate compounds by class and within each class using two or more sequential elution modes. The aim of this work was to validate a novel SE-LC method used to separate weak acids, permanently charged ions (anions), and neutral compounds by applying first a low pH isocratic elution to elute the weak acids, then an acetonitrile gradient to elute the neutral compounds, and lastly a sodium methanesulfonate gradient to elute the anionic compounds using a tandem-column arrangement comprised of a C18 column and a strong anion exchange column. The effects of flow rate, starting mobile phase organic content, column temperature, mobile phase composition, and gradient start time were investigated using a 2<sup>5-1</sup> fractional factorial design with center points for robustness. The method was demonstrated to be robust, specific, linear, accurate, and precise (repeatable) over the range of 6%–120% of the target analyte concentrations.</p>\\n </div>\",\"PeriodicalId\":17098,\"journal\":{\"name\":\"Journal of separation science\",\"volume\":\"48 3\",\"pages\":\"\"},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2025-03-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of separation science\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/jssc.70124\",\"RegionNum\":3,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of separation science","FirstCategoryId":"5","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jssc.70124","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Validation of a High-Performance Liquid Chromatography Separation of Weak Acids, Neutral Compounds, and Permanent Ions Using Sequential Elution with Tandem-Column Liquid Chromatography
Sequential elution-liquid chromatography (SE-LC) employs conventional high-performance LC instrumentation to separate compounds by class and within each class using two or more sequential elution modes. The aim of this work was to validate a novel SE-LC method used to separate weak acids, permanently charged ions (anions), and neutral compounds by applying first a low pH isocratic elution to elute the weak acids, then an acetonitrile gradient to elute the neutral compounds, and lastly a sodium methanesulfonate gradient to elute the anionic compounds using a tandem-column arrangement comprised of a C18 column and a strong anion exchange column. The effects of flow rate, starting mobile phase organic content, column temperature, mobile phase composition, and gradient start time were investigated using a 25-1 fractional factorial design with center points for robustness. The method was demonstrated to be robust, specific, linear, accurate, and precise (repeatable) over the range of 6%–120% of the target analyte concentrations.
期刊介绍：
The Journal of Separation Science (JSS) is the most comprehensive source in separation science, since it covers all areas of chromatographic and electrophoretic separation methods in theory and practice, both in the analytical and in the preparative mode, solid phase extraction, sample preparation, and related techniques. Manuscripts on methodological or instrumental developments, including detection aspects, in particular mass spectrometry, as well as on innovative applications will also be published. Manuscripts on hyphenation, automation, and miniaturization are particularly welcome. Pre- and post-separation facets of a total analysis may be covered as well as the underlying logic of the development or application of a method.
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