The Interactions Between Polyhydroxyalkanoates and Cells Involved in the Process of Tissue Damage Repair

The control of the surface topography of biomaterials is a key factor for the management of post-implantation recovery processes. The study of the influence of the nanorelief of polyhydroxyalkanoate (PHA) films on the functional state of cells involved in tissue healing process is important for the development of biocompatible materials for tissue engineering. In this work, polymer films based on polyhydroxyalkanoates of various compositions with different surface relief were analyzed. The effect of the nanorelief of PHA films on the morphology, adhesion and proliferation of cells involved in regenerative processes in tissue damage (erythrocytes, macrophages, fibroblasts) was evaluated. According to the obtained results, it was found that all films samples based on polyhydroxyalkanoates of various compositions have high biocompatibility and do not cause any pronounced negative cellular reactions. However, differences in cell morphology, adhesion, and proliferation were noted between film samples. In the case of P3HB films, a slight activation of local inflammatory reactions was noted. Thus, P3HB matrix is less preferable for use in restoration of soft tissues. On the other hand, films based on P3HB3HV demonstrated high biocompatibility, which allows to conclude that this copolymer matrix is promising for use in tissue engineering.