摘要
芦荟中含有多种矿物质、维生素和矿物质，具有润肤、通便、抗菌、消炎、抗氧化、壮阳、密封、抗真菌、防腐和美容保健价值，因为其中含有多种生物活性成分。鉴于这些特性，我们认为有必要通过对溶液中的提取物进行化学和物理化学研究来进一步研究其药用功效。因此，在当前的研究项目中，我们旨在通过从物理化学角度研究芦荟提取物的各种溶液特性，探索其药用特性/潜力，并探索其提取物与一些生物大分子（如维生素 C 和脱氧核糖核酸 (DNA)）的相互作用。首先，采集芦荟植物的叶子，然后制备其酒精提取物。然后，研究了提取物在溶液中的各种理化性质，以及它与抗坏血酸和 DNA 的相互作用。在不同浓度和温度下，通过密度、粘度、表面张力和紫外-可见光谱等测量方法对溶液中的这些参数进行了研究。在对含有和不含 DNA 的提取物水溶液进行理化研究时，在不同温度下使用了不同浓度的提取物溶液，如 0.5-5%。在理化测量中，使用含有 50 µM DNA 的溶液作为溶剂，同时改变混合物中提取物的浓度。然而，在进行紫外可见光测量时，提取物的浓度保持在 1%，而 DNA 的浓度则在 20-200 µM 的范围内变化。抗坏血酸与提取物的相互作用研究也采用了类似的方法。根据研究结果，可以说萃取物与 DNA 和/或维生素 C 的结合是物理化学性质的，主要的结合力是粗植物萃取物中化合物的氧/OH 基团与 DNA 单位的氢之间的氢键作用。
The Aloe vera contain numerous minerals, vitamins and minerals with emollient, laxative, antibacterial, anti-inflammatory, antioxidant, aphrodisiac, hermetic, antifungal, antiseptic and cosmetic health care values due to the presence of many biologically active constituents. Because of these properties, it is considered necessary to further investigate its medicinal efficacy through chemical and physicochemical studies of extracts in solution. Therefore, in the current research project we aimed to explore the medicinal characteristics/potential of the extract of Aloe vera by studying various solution properties from physicochemical point of view and to explore the interactions of its extract with some biomolecules such as Vitamin C and Deoxyribonucleic acid (DNA). First, the leaves of Aloe Vera plant were collected and then their alcoholic extract was prepared. Then, the extract was studied for its various physicochemical properties in solutions, and its interaction with Ascorbic acid and DNA. These parameters were investigated in solutions by employing density, viscosity, surface tension and UV-Visible spectroscopy etc., measurements at various concentrations and temperatures. For the physicochemical studies of the aqueous solutions of extract, with and without DNA, various concentrations of extract solutions such as 0.5‒5% were used at various temperatures. In case physicochemical measurements, a solution containing 50 µM DNA was used as solvent while the concentration of extract in the mixture was varied. However, the UV-Visible procedure was carried out by maintaining the extract concentration at 1% while altering the DNA concentration in the range of 20‒200 µM. A similar approach was also employed for the interaction study of Ascorbic acid with extract. Based on the outcomes, it may be said that the binding of the extract with DNA and/or Vitamin C be of physicochemical nature and the dominating binding force be of hydrogen bonding between the oxygen/OH groups of the compounds present in the crude plant extract and hydrogen of DNA units.
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