Maria Chiara Domini, Tommaso Castroflorio, Andrea Deregibus, Serena Ravera, Giuseppe Migliaretti, Massimo Costalonga
{"title":"正畸牙齿矫正器运动时牙龈沟液的质子核磁共振代谢组学研究。","authors":"Maria Chiara Domini, Tommaso Castroflorio, Andrea Deregibus, Serena Ravera, Giuseppe Migliaretti, Massimo Costalonga","doi":"10.1111/ocr.12916","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objectives</h3>\n \n <p>To determine the correlation between orthodontic tooth movement and a pre-defined set of gingival crevicular fluid (GCF) metabolites through proton nuclear magnetic resonance (1H-NMR) spectroscopy.</p>\n </section>\n \n <section>\n \n <h3> Materials and Methods</h3>\n \n <p>A clinical randomised prospective split-mouth study comparing the GCF metabolites around stationary and moving second maxillary molars. Twenty-four healthy subjects diagnosed with dental class II malocclusion undergoing orthodontic clear aligner treatment (CAT) were enrolled. GCF samples from the mesial and distal sulcus of second molars under stationary conditions or under 1 N of distalising force were harvested at baseline, 1 h, 7 days and 21 days after the application of CAT. 1H-NMR was utilised for GCF sample analysis. The 2-dimensional total correlation spectroscopy spectral signature of 35 known GCF metabolites was compared in moving and stationary teeth. Principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), variable importance in projection (VIP) score and area under the curve (AUC) were computed utilising MetaboAnalyst 5.0 software.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>VIP-score values showed statistically significant differences between the metabolites involved in moving and stationary molars (<i>p</i> < 0.05). PCA and PLS-DA results showed potential differences between the metabolite clusters. The variation of the 1H-NMR signals of Glutamine, Uracil, N-Acetylneuraminate and alpha-ketoglutarate contributes primarily to the variance across metabolites in moving versus stationary teeth at 1 h, 7 days and 21 days.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>High values of Glutamine and low values of Uracil, N-Acetylneurinamate and alpha-ketoglutarate could be utilised to predict the progress of orthodontic tooth movement over time. Knowledge of metabolites predictive of tooth movement could contribute to the design of tailored orthodontic treatment planning, reducing time, costs and side-effects.</p>\n </section>\n </div>","PeriodicalId":19652,"journal":{"name":"Orthodontics & Craniofacial Research","volume":"28 4","pages":"670-679"},"PeriodicalIF":1.7000,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ocr.12916","citationCount":"0","resultStr":"{\"title\":\"Proton-Nuclear Magnetic Resonance Metabolomics of Gingival Crevicular Fluid During Orthodontic Tooth Movement With Aligners\",\"authors\":\"Maria Chiara Domini, Tommaso Castroflorio, Andrea Deregibus, Serena Ravera, Giuseppe Migliaretti, Massimo Costalonga\",\"doi\":\"10.1111/ocr.12916\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Objectives</h3>\\n \\n <p>To determine the correlation between orthodontic tooth movement and a pre-defined set of gingival crevicular fluid (GCF) metabolites through proton nuclear magnetic resonance (1H-NMR) spectroscopy.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Materials and Methods</h3>\\n \\n <p>A clinical randomised prospective split-mouth study comparing the GCF metabolites around stationary and moving second maxillary molars. Twenty-four healthy subjects diagnosed with dental class II malocclusion undergoing orthodontic clear aligner treatment (CAT) were enrolled. GCF samples from the mesial and distal sulcus of second molars under stationary conditions or under 1 N of distalising force were harvested at baseline, 1 h, 7 days and 21 days after the application of CAT. 1H-NMR was utilised for GCF sample analysis. The 2-dimensional total correlation spectroscopy spectral signature of 35 known GCF metabolites was compared in moving and stationary teeth. Principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), variable importance in projection (VIP) score and area under the curve (AUC) were computed utilising MetaboAnalyst 5.0 software.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>VIP-score values showed statistically significant differences between the metabolites involved in moving and stationary molars (<i>p</i> < 0.05). PCA and PLS-DA results showed potential differences between the metabolite clusters. The variation of the 1H-NMR signals of Glutamine, Uracil, N-Acetylneuraminate and alpha-ketoglutarate contributes primarily to the variance across metabolites in moving versus stationary teeth at 1 h, 7 days and 21 days.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>High values of Glutamine and low values of Uracil, N-Acetylneurinamate and alpha-ketoglutarate could be utilised to predict the progress of orthodontic tooth movement over time. 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Proton-Nuclear Magnetic Resonance Metabolomics of Gingival Crevicular Fluid During Orthodontic Tooth Movement With Aligners
Objectives
To determine the correlation between orthodontic tooth movement and a pre-defined set of gingival crevicular fluid (GCF) metabolites through proton nuclear magnetic resonance (1H-NMR) spectroscopy.
Materials and Methods
A clinical randomised prospective split-mouth study comparing the GCF metabolites around stationary and moving second maxillary molars. Twenty-four healthy subjects diagnosed with dental class II malocclusion undergoing orthodontic clear aligner treatment (CAT) were enrolled. GCF samples from the mesial and distal sulcus of second molars under stationary conditions or under 1 N of distalising force were harvested at baseline, 1 h, 7 days and 21 days after the application of CAT. 1H-NMR was utilised for GCF sample analysis. The 2-dimensional total correlation spectroscopy spectral signature of 35 known GCF metabolites was compared in moving and stationary teeth. Principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), variable importance in projection (VIP) score and area under the curve (AUC) were computed utilising MetaboAnalyst 5.0 software.
Results
VIP-score values showed statistically significant differences between the metabolites involved in moving and stationary molars (p < 0.05). PCA and PLS-DA results showed potential differences between the metabolite clusters. The variation of the 1H-NMR signals of Glutamine, Uracil, N-Acetylneuraminate and alpha-ketoglutarate contributes primarily to the variance across metabolites in moving versus stationary teeth at 1 h, 7 days and 21 days.
Conclusion
High values of Glutamine and low values of Uracil, N-Acetylneurinamate and alpha-ketoglutarate could be utilised to predict the progress of orthodontic tooth movement over time. Knowledge of metabolites predictive of tooth movement could contribute to the design of tailored orthodontic treatment planning, reducing time, costs and side-effects.
期刊介绍：
Orthodontics & Craniofacial Research - Genes, Growth and Development is published to serve its readers as an international forum for the presentation and critical discussion of issues pertinent to the advancement of the specialty of orthodontics and the evidence-based knowledge of craniofacial growth and development. This forum is based on scientifically supported information, but also includes minority and conflicting opinions.
The objective of the journal is to facilitate effective communication between the research community and practicing clinicians. Original papers of high scientific quality that report the findings of clinical trials, clinical epidemiology, and novel therapeutic or diagnostic approaches are appropriate submissions. Similarly, we welcome papers in genetics, developmental biology, syndromology, surgery, speech and hearing, and other biomedical disciplines related to clinical orthodontics and normal and abnormal craniofacial growth and development. In addition to original and basic research, the journal publishes concise reviews, case reports of substantial value, invited essays, letters, and announcements.
The journal is published quarterly. The review of submitted papers will be coordinated by the editor and members of the editorial board. It is policy to review manuscripts within 3 to 4 weeks of receipt and to publish within 3 to 6 months of acceptance.
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