Somaye Shiralizadeh, Abbas Farmani, Leili Shokoohizadeh, Maryam Pourhajibagher, Mohammad Yousef Alikhani, Abbas Bahador
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The synthesised CDs were analysed by HRTEM, zeta potential, UV-Vis, FTIR and fluorescence. The efficacy of photodynamic therapy (PDT) against P. aeruginosa ATCC27853 and two clinical P. aeruginosa isolates was evaluated using a combination of sub-minimum inhibitory concentrations (sub-MIC) and sub-lethal doses of UVA light (sDL) irradiation. The nanoscale size, electrical charge and photophysical properties of the synthesised CDs were confirmed by HRTEM, zeta potential, UV-Vis, FTIR and fluorescence techniques. Treatment with CDsGEN-NH2, CDsIMP-NH2 and CDsGEN-IMP at concentrations of 3 mg/mL (sub-MIC) and sDL irradiation for 10 s (3.12 J/cm<sup>2</sup>) resulted in significantly reduced viability in P. aeruginosa ATCC27853 compared to the control (P ≤ 0.05), achieving viable cell reductions of 3.44, 3.11 and 2.65 log, respectively. 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引用次数: 0
摘要
日益普遍的抗生素耐药性对卫生保健系统管理铜绿假单胞菌感染的能力构成了重大威胁，突出了创新治疗策略的必要性。本研究的目的是评价庆大霉素和亚胺培南碳点光动力治疗（cd）对铜绿假单胞菌（P. aeruginosa）分离株的效果。采用水热法分别以庆大霉素和亚胺培南为前体合成胺功能化CDs （CDsGEN-NH2, CDsIMP-NH2）。用N-（3-二甲氨基丙基）-N′-乙基碳二亚胺盐酸盐和N-羟基琥珀酰亚胺实现亚胺培南与CDsGEN-NH2 （CDsGEN-IMP）和庆大霉素与CDsIMP-NH2 （CDsIMP-GEN）的偶联。采用HRTEM、zeta电位、UV-Vis、FTIR和荧光等方法对合成的CDs进行了分析。光动力疗法（PDT）对铜绿假单胞菌ATCC27853和两个临床铜绿假单胞菌分离株的疗效通过亚最低抑制浓度（亚mic）和亚致死剂量的UVA光（sDL）照射进行评估。利用HRTEM、zeta电位、UV-Vis、FTIR和荧光技术对合成CDs的纳米级尺寸、电荷和光物理性质进行了表征。CDsGEN-NH2、CDsIMP-NH2和CDsGEN-IMP在浓度为3 mg/mL（亚mic）和sDL照射10 s （3.12 J/cm2）的情况下，与对照组相比，P. aeruginosa ATCC27853的活力显著降低（P≤0.05），活细胞减少量分别为3.44、3.11和2.65 log。CDsIMP-GEN浓度为125µg/mL（亚mic）， sDL照射10 s （3.12 J/cm2）也显著降低了铜绿假单胞菌ATCC27853的活力（P≤0.05），使活细胞减少3.75 log。这些结果表明，亚胺培南和庆大霉素衍生的CDs可作为有效的抗菌药物与UVA光照射联合用于抗菌光动力治疗。
The effect of photodynamic therapy using gentamicin and imipenem-derived carbon dots on Pseudomonas aeruginosa isolates.
The increasing prevalence of antibiotic resistance poses a significant threat to the ability of healthcare systems to manage Pseudomonas aeruginosa infections, highlighting the need for innovative therapeutic strategies. The aim of this study was to evaluate the effects of photodynamic therapy (PDT) using carbon dots (CDs) derived from gentamicin and imipenem against P. aeruginosa isolates. A hydrothermal method was used to synthesise amine-functionalised CDs (CDsGEN-NH2, CDsIMP-NH2) from gentamicin and imipenem precursors, respectively. Conjugation of imipenem to CDsGEN-NH2 (CDsGEN-IMP) and gentamicin to CDsIMP-NH2 (CDsIMP-GEN) was achieved using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide. The synthesised CDs were analysed by HRTEM, zeta potential, UV-Vis, FTIR and fluorescence. The efficacy of photodynamic therapy (PDT) against P. aeruginosa ATCC27853 and two clinical P. aeruginosa isolates was evaluated using a combination of sub-minimum inhibitory concentrations (sub-MIC) and sub-lethal doses of UVA light (sDL) irradiation. The nanoscale size, electrical charge and photophysical properties of the synthesised CDs were confirmed by HRTEM, zeta potential, UV-Vis, FTIR and fluorescence techniques. Treatment with CDsGEN-NH2, CDsIMP-NH2 and CDsGEN-IMP at concentrations of 3 mg/mL (sub-MIC) and sDL irradiation for 10 s (3.12 J/cm2) resulted in significantly reduced viability in P. aeruginosa ATCC27853 compared to the control (P ≤ 0.05), achieving viable cell reductions of 3.44, 3.11 and 2.65 log, respectively. Treatment with CDsIMP-GEN at a concentration of 125 µg/mL (sub-MIC) and sDL irradiation for 10 s (3.12 J/cm2) also significantly reduced viability in P. aeruginosa ATCC27853 compared to the control (P ≤ 0.05), achieving a viable cell reduction of 3.75 log. These results demonstrate that CDs derived from imipenem and gentamicin can serve as effective antimicrobial agents in combination with UVA light irradiation for antimicrobial photodynamic therapy.
期刊介绍：
Lasers in Medical Science (LIMS) has established itself as the leading international journal in the rapidly expanding field of medical and dental applications of lasers and light. It provides a forum for the publication of papers on the technical, experimental, and clinical aspects of the use of medical lasers, including lasers in surgery, endoscopy, angioplasty, hyperthermia of tumors, and photodynamic therapy. In addition to medical laser applications, LIMS presents high-quality manuscripts on a wide range of dental topics, including aesthetic dentistry, endodontics, orthodontics, and prosthodontics.
The journal publishes articles on the medical and dental applications of novel laser technologies, light delivery systems, sensors to monitor laser effects, basic laser-tissue interactions, and the modeling of laser-tissue interactions. Beyond laser applications, LIMS features articles relating to the use of non-laser light-tissue interactions.
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