Göksu Özçelikay Akyildiz, Mehmet Altay Ünal, Seçil Gülden, Sibel A Özkan
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引用次数: 0
摘要
性别决定区Y-box 2 (SOX2)是一种转录因子蛋白。SOX2表达与结直肠癌淋巴结转移和远处转移有关。用第一个磁性一次性免疫平台测定SOX2。所设计的三明治状免疫复合物由捕获抗体、SOX2蛋白和生物素化二抗(dAb/HRP)组成。三明治形状的免疫复合物与羧酸功能化磁珠(hooc - mb)相连。将这种磁性生物偶联物滴在丝网印刷碳电极(SPCE)表面。在对苯二酚(HQ)和H2O2存在下,在-0.20 V下对银伪参比电极进行安培测量。评价了影响免疫测定反应的优化参数。对一次性磁性免疫平台用于SOX2标准品的安培检测进行了分析评价。该免疫传感器灵敏度高(LOD为1.37 ng mL-1),分析时间短(15 min)。测试了血清样本中发现的潜在干扰化合物。评价磁性一次性免疫平台的贮存稳定性。将所研制的免疫传感器与ELISA法进行比较。
The magnetic beads-based sandwich-shaped immune complexes for rapid and sensitive amperometric detection of SOX2 protein.
Sex-Determining Region Y-box 2 (SOX2) is a transcription factor protein. SOX2 expression is related to lymph node metastasis and distant metastasis in colorectal carcinomas. SOX2 was determined with the first magnetic disposable immunoplatform. The designed sandwich-shaped immune complexes were formed by a capture antibody, SOX2 protein, and biotinylated secondary antibodies (dAb/HRP). The sandwich-shaped immune complex was linked to carboxylic acid functionalized magnetic beads (HOOC-MBs). This magnetic bioconjugate was dropped on the surface of the screen-printed carbon electrode (SPCE). The amperometric measurement was performed at -0.20 V in the presence of hydroquinone (HQ) and H2O2 against a silver pseudo-reference electrode. The optimization parameters affecting the immunoassay response were evaluated. The analytical evaluation of the magnetic disposable immunoplatform for the amperometric detection of SOX2 standards was done. The developed immunosensor shows high sensitivity (LOD of 1.37 ng mL-1) and a short analysis time (15 min). Potential interfering compounds found in serum samples were tested. The storage stability of magnetic disposable immunoplatform was evaluated. The developed immunosensor was compared with the ELISA method.
期刊介绍:
The Turkish Journal of Chemistry is a bimonthly multidisciplinary journal published by the Scientific and Technological Research Council of Turkey (TÜBİTAK).
The journal is dedicated to dissemination of knowledge in all disciplines of chemistry (organic, inorganic, physical, polymeric, technical, theoretical and analytical chemistry) as well as research at the interface with other sciences especially in chemical engineering where molecular aspects are key to the findings.
The journal accepts English-language original manuscripts and contribution is open to researchers of all nationalities.
The journal publishes refereed original papers, reviews, letters to editor and issues devoted to special fields.
All manuscripts are peer-reviewed and electronic processing ensures accurate reproduction of text and data, plus publication times as short as possible.