基于磁珠的夹心形免疫复合物,用于快速灵敏地检测 SOX2 蛋白。

IF 1.3 4区 化学 Q3 CHEMISTRY, MULTIDISCIPLINARY
Turkish Journal of Chemistry Pub Date : 2024-12-16 eCollection Date: 2025-01-01 DOI:10.55730/1300-0527.3712
Göksu Özçelikay Akyildiz, Mehmet Altay Ünal, Seçil Gülden, Sibel A Özkan
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引用次数: 0

摘要

本文章由计算机程序翻译,如有差异,请以英文原文为准。
The magnetic beads-based sandwich-shaped immune complexes for rapid and sensitive amperometric detection of SOX2 protein.

Sex-Determining Region Y-box 2 (SOX2) is a transcription factor protein. SOX2 expression is related to lymph node metastasis and distant metastasis in colorectal carcinomas. SOX2 was determined with the first magnetic disposable immunoplatform. The designed sandwich-shaped immune complexes were formed by a capture antibody, SOX2 protein, and biotinylated secondary antibodies (dAb/HRP). The sandwich-shaped immune complex was linked to carboxylic acid functionalized magnetic beads (HOOC-MBs). This magnetic bioconjugate was dropped on the surface of the screen-printed carbon electrode (SPCE). The amperometric measurement was performed at -0.20 V in the presence of hydroquinone (HQ) and H2O2 against a silver pseudo-reference electrode. The optimization parameters affecting the immunoassay response were evaluated. The analytical evaluation of the magnetic disposable immunoplatform for the amperometric detection of SOX2 standards was done. The developed immunosensor shows high sensitivity (LOD of 1.37 ng mL-1) and a short analysis time (15 min). Potential interfering compounds found in serum samples were tested. The storage stability of magnetic disposable immunoplatform was evaluated. The developed immunosensor was compared with the ELISA method.

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来源期刊
Turkish Journal of Chemistry
Turkish Journal of Chemistry 化学-工程:化工
CiteScore
2.40
自引率
7.10%
发文量
87
审稿时长
3 months
期刊介绍: The Turkish Journal of Chemistry is a bimonthly multidisciplinary journal published by the Scientific and Technological Research Council of Turkey (TÜBİTAK). The journal is dedicated to dissemination of knowledge in all disciplines of chemistry (organic, inorganic, physical, polymeric, technical, theoretical and analytical chemistry) as well as research at the interface with other sciences especially in chemical engineering where molecular aspects are key to the findings. The journal accepts English-language original manuscripts and contribution is open to researchers of all nationalities. The journal publishes refereed original papers, reviews, letters to editor and issues devoted to special fields. All manuscripts are peer-reviewed and electronic processing ensures accurate reproduction of text and data, plus publication times as short as possible.
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