干扰素调节因子3(IRF3)基因在穆图鲁牛、白富拉尼牛和恩达马牛中的多态性鉴定和遗传多样性研究。

IF 1.7 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Abubakar A Mohammed, Michael O Ozoje, John S De Campos, Christian O Ikeobi, Adewale O Talabi, Samuel O Durosaro, Babatunde M Ilori, Johnson A Aderoju
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引用次数: 0

摘要

干扰素调节因子3 (IRF3)是与许多健康相关表型(如一般抗病)相关的最强位置候选基因之一。该研究对恩达马牛、穆图鲁牛和白富拉尼牛的IRF3基因进行了遗传表征。使用Zymo-spin提取试剂盒从血液样本中提取DNA。使用ARLEQUIN 2.0001软件估计基本群体遗传统计量,使用DnaSP 5.10.01版本估计遗传多样性指数和检验偏离中性。经聚合酶链反应和测序,在3个牛品种中分别在IRF3基因外显子1 ~ 2和5 ~ 6处发现了18和29个单核苷酸多态性(snp)。在外显子1 ~ 2中,Muturu (MU)拥有最多的snp(16个)和遗传多样性指数,而N’dama (ND)拥有最少的snp(1个)。在外显子5 ~ 6中,白富拉尼人拥有最多的snp(14个),遗传多样性指数也很高,Muturu (Muturu)最少。对所考虑的位点进行的分子变异分析(AMOVA)显示,群体间的变异水平高于群体内的变异水平。因此,IRF3基因在尼日利亚牛品种中具有多种多态性和高度多样化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification of polymorphisms and genetic diversity studies of interferon regulatory factor 3(IRF3) gene in Muturu,White Fulani and N'Dama cattle.

Interferon regulatory factor 3 (IRF3) is one of the strongest positional candidate genes implicated in a host of health-related phenotypes such as general disease resistance. The study was carried out to genetically characterize IRF3 gene in, N'Dama, Muturu and White Fulani cattle. DNA was extracted from the blood samples using the Zymo-spin extraction kit. ARLEQUIN 2.0001 software was used to estimate the basic population genetic statistics while DnaSP version 5.10.01 was used to estimate genetic diversity indices and test for deviation from neutrality. A total of 18 and 29 Single nucleotide polymorphisms (SNPs) were observed using codon code aligner in exons 1-2 and 5-6 of the IRF3 gene respectively in the three cattle breeds after polymerase chain reaction and sequencing. In exons 1-2, the Muturu (MU) possessed the highest value of SNPs (16) and genetic diversity indices, while the N'Dama (ND) possessed the least (1). In exons 5-6, the highest value of SNPs (14) was observed in the White Fulani and the genetic diversity indices was also high while the Muturu had the least. Analysis of molecular variation (AMOVA) carried out for the loci under consideration revealed a higher level of variation among populations than within populations. It was therefore concluded that the IRF3 gene had many polymorphisms and was highly diversified in Nigerian cattle breeds.

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来源期刊
Tropical animal health and production
Tropical animal health and production 农林科学-兽医学
CiteScore
3.40
自引率
11.80%
发文量
361
审稿时长
6-12 weeks
期刊介绍: Tropical Animal Health and Production is an international journal publishing the results of original research in any field of animal health, welfare, and production with the aim of improving health and productivity of livestock, and better utilisation of animal resources, including wildlife in tropical, subtropical and similar agro-ecological environments.
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