生长激素和GnRH在雄性大鼠全坐骨神经横断模型中的神经营养作用。

IF 3.2 2区 医学 Q2 ENDOCRINOLOGY & METABOLISM
Jorge J A Baca-Alonso, Denisse Calderón-Vallejo, Irma Hernández-Jasso, David Epardo, Jerusa E Balderas-Márquez, Maricela Luna, Carlos Arámburo, J Luis Quintanar, Carlos G Martínez-Moreno
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引用次数: 0

摘要

周围神经损伤,如坐骨神经横断(SNT),与显著的感觉和运动缺陷有关。生长激素(GH)和促性腺激素释放激素(GnRH)已被证明发挥神经营养作用,可以促进神经再生和功能神经再生。然而,这些激素对周围神经修复的综合影响仍然知之甚少。本研究旨在分析GH和GnRH在切除睾丸的雄性大鼠SNT模型中的单独和联合作用,以阻止激素介导的神经再生和神经保护。治疗包括GH、GnRH或两者结合,随后评估运动和感觉功能,以及神经组织和相关肌肉的组织学和分子分析。结果显示,单独给药时,GH和GnRH均能显著增强神经再生和神经功能。治疗后的动物表现出轴突生长、髓鞘形成以及感觉和运动功能恢复的改善。此外,GH和GnRH减少神经炎症/反应性胶质瘤,这可以通过下调TNFα IL-1β、Iba-1和GFAP来证明,这些物质通常在神经损伤后升高。这些发现表明,每一种激素独立支持损伤后神经修复和功能恢复的关键方面。令人惊讶的是,当生长激素和GnRH同时使用时,它们的有益效果并不是相加的。相反,与单独治疗相比,两种治疗相结合导致的结果减少。具体来说,接受联合治疗的动物显示轴突组织减少,髓鞘受损,功能改善较少。总之,GH和GnRH显示出作为促进神经再生的单独治疗药物的潜力,在轴突生长、功能恢复和减少神经炎症方面都有显著的益处。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Neurotrophic effects of GH and GnRH in a full sciatic nerve transection model in male rats.

Peripheral nerve injuries, such as sciatic nerve transection (SNT), are associated with significant sensory and motor deficits. Growth hormone (GH) and gonadotropin-releasing hormone (GnRH) have been shown to exert neurotrophic effects that can promote nerve regeneration and functional reinnervation. However, the combined impact of these hormones on peripheral nerve repair remains poorly understood. This study aimed to analyze the individual and combined effects of GH and GnRH in a rat model of SNT, using orchiectomized male rats to prevent steroid-mediated neuroregeneration and neuroprotection. Treatments included GH, GnRH, or a combination of both, with subsequent assessments of motor and sensory function, as well as histological and molecular analyses of the nerve tissue and associated muscles. The results revealed that both GH and GnRH significantly enhanced nerve regeneration and neural function when administered individually. Treated animals exhibited improved axonal growth, myelination, as well as sensory and motor functional recovery. In addition, GH and GnRH reduced neuroinflammation/reactive gliosis, as evidenced by the downregulation of TNFα IL-1β, Iba-1 and GFAP, which are typically elevated following nerve injury. These findings indicate that each hormone independently supports critical aspects of nerve repair and functional restoration after injury. Surprisingly, when GH and GnRH were administered together, their beneficial effects were not additive. Instead, the combination of the two treatments led to diminished outcomes in comparison to either treatment alone. Specifically, animals receiving the combined therapy showed reduced axonal organization, impaired myelination, and less functional improvement. In conclusion, GH and GnRH demonstrate potential as individual therapeutic agents for promoting nerve regeneration, each providing significant benefits in terms of axonal growth, functional recovery, and reduction of neuroinflammation.

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来源期刊
Neuroendocrinology
Neuroendocrinology 医学-内分泌学与代谢
CiteScore
8.30
自引率
2.40%
发文量
50
审稿时长
6-12 weeks
期刊介绍: ''Neuroendocrinology'' publishes papers reporting original research in basic and clinical neuroendocrinology. The journal explores the complex interactions between neuronal networks and endocrine glands (in some instances also immunecells) in both central and peripheral nervous systems. Original contributions cover all aspects of the field, from molecular and cellular neuroendocrinology, physiology, pharmacology, and the neuroanatomy of neuroendocrine systems to neuroendocrine correlates of behaviour, clinical neuroendocrinology and neuroendocrine cancers. Readers also benefit from reviews by noted experts, which highlight especially active areas of current research, and special focus editions of topical interest.
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