{"title":"杀菌剂种子处理延缓了冬小麦丛枝菌根真菌在温室内的定植,但在田间效果减弱。","authors":"Hardy Brieuc, Belvaux Eléonore, Huyghebaert Bruno, Declerck Stéphane, Calonne-Salmon Maryline","doi":"10.1007/s00572-025-01199-3","DOIUrl":null,"url":null,"abstract":"<p><p>Seed-applied fungicides support agricultural production by controlling seed- or soil-borne diseases. However, they can impact non-target soil organisms. In this study, we investigated the effect of eight seed treatments (including two authorized for organic farming) on root colonization of winter wheat (Triticum aestivum L.) by arbuscular mycorrhizal (AM) fungi. One experiment was conducted in greenhouse conditions, on a sterile substrate inoculated with the AM fungus Rhizophagus irregularis MUCL 41833 and one in field conditions, where winter wheat was colonized by native soil AM fungi. In greenhouse conditions, the six conventional seed treatments reduced root colonization five weeks after sowing. No difference with the control treatment was measured thereafter for a product containing triazole alone. In contrast, seed treatments containing fludioxonil (fungicide molecule alone or formulated with the triazole difenoconazole), and prochloraz formulated with the triazole triticonazole significantly reduced root colonization until 11 weeks after sowing. Notably, when formulated with sedaxane, the adverse effect of fludioxonil was reduced. The negative effect of seed treatments on AM fungal root colonization in field was smaller than in the greenhouse and generally not significant, with disparate results from one timestep to another. This may be related to the dilution or the degradation of the active ingredients in the soil during the winter period or AM fungal species/strain involved in symbiosis. Overall, our results outline that the direct effect of seed treatment is highly variable depending on the modes of action, half-lives and interactions between active ingredients. By contributing to highlight the undesired effects of pesticides on AM fungi (i.e., by delaying root colonization), this study pleads for a reduction of pesticide applications to encourage the rapid and efficient establishment of functional mycorrhizal symbioses.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"35 2","pages":"22"},"PeriodicalIF":3.8000,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Fungicide seed treatments delay arbuscular mycorrhizal fungi colonization of winter wheat in the greenhouse, but the effect is attenuated in the field.\",\"authors\":\"Hardy Brieuc, Belvaux Eléonore, Huyghebaert Bruno, Declerck Stéphane, Calonne-Salmon Maryline\",\"doi\":\"10.1007/s00572-025-01199-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Seed-applied fungicides support agricultural production by controlling seed- or soil-borne diseases. However, they can impact non-target soil organisms. In this study, we investigated the effect of eight seed treatments (including two authorized for organic farming) on root colonization of winter wheat (Triticum aestivum L.) by arbuscular mycorrhizal (AM) fungi. One experiment was conducted in greenhouse conditions, on a sterile substrate inoculated with the AM fungus Rhizophagus irregularis MUCL 41833 and one in field conditions, where winter wheat was colonized by native soil AM fungi. In greenhouse conditions, the six conventional seed treatments reduced root colonization five weeks after sowing. No difference with the control treatment was measured thereafter for a product containing triazole alone. In contrast, seed treatments containing fludioxonil (fungicide molecule alone or formulated with the triazole difenoconazole), and prochloraz formulated with the triazole triticonazole significantly reduced root colonization until 11 weeks after sowing. Notably, when formulated with sedaxane, the adverse effect of fludioxonil was reduced. The negative effect of seed treatments on AM fungal root colonization in field was smaller than in the greenhouse and generally not significant, with disparate results from one timestep to another. This may be related to the dilution or the degradation of the active ingredients in the soil during the winter period or AM fungal species/strain involved in symbiosis. Overall, our results outline that the direct effect of seed treatment is highly variable depending on the modes of action, half-lives and interactions between active ingredients. By contributing to highlight the undesired effects of pesticides on AM fungi (i.e., by delaying root colonization), this study pleads for a reduction of pesticide applications to encourage the rapid and efficient establishment of functional mycorrhizal symbioses.</p>\",\"PeriodicalId\":18965,\"journal\":{\"name\":\"Mycorrhiza\",\"volume\":\"35 2\",\"pages\":\"22\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2025-03-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mycorrhiza\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s00572-025-01199-3\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MYCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycorrhiza","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00572-025-01199-3","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MYCOLOGY","Score":null,"Total":0}
Fungicide seed treatments delay arbuscular mycorrhizal fungi colonization of winter wheat in the greenhouse, but the effect is attenuated in the field.
Seed-applied fungicides support agricultural production by controlling seed- or soil-borne diseases. However, they can impact non-target soil organisms. In this study, we investigated the effect of eight seed treatments (including two authorized for organic farming) on root colonization of winter wheat (Triticum aestivum L.) by arbuscular mycorrhizal (AM) fungi. One experiment was conducted in greenhouse conditions, on a sterile substrate inoculated with the AM fungus Rhizophagus irregularis MUCL 41833 and one in field conditions, where winter wheat was colonized by native soil AM fungi. In greenhouse conditions, the six conventional seed treatments reduced root colonization five weeks after sowing. No difference with the control treatment was measured thereafter for a product containing triazole alone. In contrast, seed treatments containing fludioxonil (fungicide molecule alone or formulated with the triazole difenoconazole), and prochloraz formulated with the triazole triticonazole significantly reduced root colonization until 11 weeks after sowing. Notably, when formulated with sedaxane, the adverse effect of fludioxonil was reduced. The negative effect of seed treatments on AM fungal root colonization in field was smaller than in the greenhouse and generally not significant, with disparate results from one timestep to another. This may be related to the dilution or the degradation of the active ingredients in the soil during the winter period or AM fungal species/strain involved in symbiosis. Overall, our results outline that the direct effect of seed treatment is highly variable depending on the modes of action, half-lives and interactions between active ingredients. By contributing to highlight the undesired effects of pesticides on AM fungi (i.e., by delaying root colonization), this study pleads for a reduction of pesticide applications to encourage the rapid and efficient establishment of functional mycorrhizal symbioses.
期刊介绍:
Mycorrhiza is an international journal devoted to research into mycorrhizas - the widest symbioses in nature, involving plants and a range of soil fungi world-wide. The scope of Mycorrhiza covers all aspects of research into mycorrhizas, including molecular biology of the plants and fungi, fungal systematics, development and structure of mycorrhizas, and effects on plant physiology, productivity, reproduction and disease resistance. The scope also includes interactions between mycorrhizal fungi and other soil organisms and effects of mycorrhizas on plant biodiversity and ecosystem structure.
Mycorrhiza contains original papers, short notes and review articles, along with commentaries and news items. It forms a platform for new concepts and discussions, and is a basis for a truly international forum of mycorrhizologists from all over the world.