{"title":"从油茶根际土壤中分离的Kitasatospora camelliae sp. nov。","authors":"Kaiqin Li, Ying Qian, Jian Gao, Wei Liang","doi":"10.1099/ijsem.0.006717","DOIUrl":null,"url":null,"abstract":"<p><p>Strain HUAS MG31<sup>T</sup> was isolated from the rhizosphere soil of <i>Camellia oleifera</i> collected from Taoyuan County, China. This strain produced white substrate mycelium and hair brown aerial mycelium without diffusible pigment on the Gause's synthetic No. 1 medium. Aerial mycelia differentiated into rectiflexible spore chains consisting of spherical or cylindrical spores with smooth surfaces. Cell wall peptidoglycan of strain HUAS MG31<sup>T</sup> contained <i>meso</i>-diaminopimelic acid, and whole-cell sugars were galactose and mannose. The menaquinones of strain HUAS MG31<sup>T</sup> were MK-9(H<sub>6</sub>), MK-9(H<sub>8</sub>) and MK-9(H<sub>4</sub>). The major cellular fatty acids consisted of Summed Feature 9 (<i>iso</i>-C<sub>17 : 1</sub> ω9c/10-methyl C<sub>16 : 0</sub>), <i>iso</i>-C<sub>16 : 0</sub>, <i>iso</i>-C<sub>15 : 0</sub>, <i>anteiso</i>-C<sub>15 : 0</sub>, <i>iso</i>-C<sub>17 : 0</sub>, <i>anteiso</i>-C<sub>17 : 0</sub>, Summed Feature 3 (<i>iso</i> H-C<sub>16 : 1</sub> /<sub>C16 : 1</sub> ω6c) and cyclo C<sub>17 : 0</sub>. Sequence analysis based on the full-length 16S rRNA gene of strain HUAS MG31<sup>T</sup> showed that it shared highest sequence similarities to <i>Kitasatospora paranensis</i> HKI 0190<sup>T</sup> (99.5%) and <i>Kitasatospora terrestris</i> HKI 0186<sup>T</sup> (99.4%). The phylogenomic tree shows that strain HUAS MG31<sup>T</sup> forms an independent subclade, indicating that it might belong to a potential novel species. The results from phylogenetic trees based on 16S rRNA gene sequences showed that strain HUAS MG31<sup>T</sup> was most closely related to <i>K. paranensis</i> HKI 0190<sup>T</sup>. However, the average nucleotide identity and the digital DNA-DNA hybridization between strain HUAS MG31<sup>T</sup> and <i>K. paranensis</i> JCM 13005<sup>T</sup> were 87.0 %/81.2% and 25.8 %, respectively, below the 95%-96% and 70 % threshold that defined a new species. Meanwhile, phenotypic, chemotaxonomic characteristics and MALDI-TOF MS results further confirmed that strain HUAS MG31<sup>T</sup> was significantly different from <i>K. paranensis</i> JCM 13005<sup>T</sup>. Therefore, these results reveal that strain HUAS MG31<sup>T</sup> represents a novel species of the genus <i>Kitasatospora</i>, for which the name <i>Kitasatospora camelliae</i> sp. nov. is proposed. The type strain is HUAS MG31<sup>T</sup> (=MCCC 1K09225<sup>T</sup>= JCM 37022<sup>T</sup>).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"<i>Kitasatospora camelliae</i> sp. nov., isolated from rhizosphere soil of <i>Camellia oleifera</i>.\",\"authors\":\"Kaiqin Li, Ying Qian, Jian Gao, Wei Liang\",\"doi\":\"10.1099/ijsem.0.006717\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Strain HUAS MG31<sup>T</sup> was isolated from the rhizosphere soil of <i>Camellia oleifera</i> collected from Taoyuan County, China. This strain produced white substrate mycelium and hair brown aerial mycelium without diffusible pigment on the Gause's synthetic No. 1 medium. Aerial mycelia differentiated into rectiflexible spore chains consisting of spherical or cylindrical spores with smooth surfaces. Cell wall peptidoglycan of strain HUAS MG31<sup>T</sup> contained <i>meso</i>-diaminopimelic acid, and whole-cell sugars were galactose and mannose. The menaquinones of strain HUAS MG31<sup>T</sup> were MK-9(H<sub>6</sub>), MK-9(H<sub>8</sub>) and MK-9(H<sub>4</sub>). The major cellular fatty acids consisted of Summed Feature 9 (<i>iso</i>-C<sub>17 : 1</sub> ω9c/10-methyl C<sub>16 : 0</sub>), <i>iso</i>-C<sub>16 : 0</sub>, <i>iso</i>-C<sub>15 : 0</sub>, <i>anteiso</i>-C<sub>15 : 0</sub>, <i>iso</i>-C<sub>17 : 0</sub>, <i>anteiso</i>-C<sub>17 : 0</sub>, Summed Feature 3 (<i>iso</i> H-C<sub>16 : 1</sub> /<sub>C16 : 1</sub> ω6c) and cyclo C<sub>17 : 0</sub>. Sequence analysis based on the full-length 16S rRNA gene of strain HUAS MG31<sup>T</sup> showed that it shared highest sequence similarities to <i>Kitasatospora paranensis</i> HKI 0190<sup>T</sup> (99.5%) and <i>Kitasatospora terrestris</i> HKI 0186<sup>T</sup> (99.4%). The phylogenomic tree shows that strain HUAS MG31<sup>T</sup> forms an independent subclade, indicating that it might belong to a potential novel species. The results from phylogenetic trees based on 16S rRNA gene sequences showed that strain HUAS MG31<sup>T</sup> was most closely related to <i>K. paranensis</i> HKI 0190<sup>T</sup>. However, the average nucleotide identity and the digital DNA-DNA hybridization between strain HUAS MG31<sup>T</sup> and <i>K. paranensis</i> JCM 13005<sup>T</sup> were 87.0 %/81.2% and 25.8 %, respectively, below the 95%-96% and 70 % threshold that defined a new species. Meanwhile, phenotypic, chemotaxonomic characteristics and MALDI-TOF MS results further confirmed that strain HUAS MG31<sup>T</sup> was significantly different from <i>K. paranensis</i> JCM 13005<sup>T</sup>. Therefore, these results reveal that strain HUAS MG31<sup>T</sup> represents a novel species of the genus <i>Kitasatospora</i>, for which the name <i>Kitasatospora camelliae</i> sp. nov. is proposed. The type strain is HUAS MG31<sup>T</sup> (=MCCC 1K09225<sup>T</sup>= JCM 37022<sup>T</sup>).</p>\",\"PeriodicalId\":14390,\"journal\":{\"name\":\"International journal of systematic and evolutionary microbiology\",\"volume\":\"75 3\",\"pages\":\"\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of systematic and evolutionary microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1099/ijsem.0.006717\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of systematic and evolutionary microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1099/ijsem.0.006717","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Kitasatospora camelliae sp. nov., isolated from rhizosphere soil of Camellia oleifera.
Strain HUAS MG31T was isolated from the rhizosphere soil of Camellia oleifera collected from Taoyuan County, China. This strain produced white substrate mycelium and hair brown aerial mycelium without diffusible pigment on the Gause's synthetic No. 1 medium. Aerial mycelia differentiated into rectiflexible spore chains consisting of spherical or cylindrical spores with smooth surfaces. Cell wall peptidoglycan of strain HUAS MG31T contained meso-diaminopimelic acid, and whole-cell sugars were galactose and mannose. The menaquinones of strain HUAS MG31T were MK-9(H6), MK-9(H8) and MK-9(H4). The major cellular fatty acids consisted of Summed Feature 9 (iso-C17 : 1 ω9c/10-methyl C16 : 0), iso-C16 : 0, iso-C15 : 0, anteiso-C15 : 0, iso-C17 : 0, anteiso-C17 : 0, Summed Feature 3 (iso H-C16 : 1 /C16 : 1 ω6c) and cyclo C17 : 0. Sequence analysis based on the full-length 16S rRNA gene of strain HUAS MG31T showed that it shared highest sequence similarities to Kitasatospora paranensis HKI 0190T (99.5%) and Kitasatospora terrestris HKI 0186T (99.4%). The phylogenomic tree shows that strain HUAS MG31T forms an independent subclade, indicating that it might belong to a potential novel species. The results from phylogenetic trees based on 16S rRNA gene sequences showed that strain HUAS MG31T was most closely related to K. paranensis HKI 0190T. However, the average nucleotide identity and the digital DNA-DNA hybridization between strain HUAS MG31T and K. paranensis JCM 13005T were 87.0 %/81.2% and 25.8 %, respectively, below the 95%-96% and 70 % threshold that defined a new species. Meanwhile, phenotypic, chemotaxonomic characteristics and MALDI-TOF MS results further confirmed that strain HUAS MG31T was significantly different from K. paranensis JCM 13005T. Therefore, these results reveal that strain HUAS MG31T represents a novel species of the genus Kitasatospora, for which the name Kitasatospora camelliae sp. nov. is proposed. The type strain is HUAS MG31T (=MCCC 1K09225T= JCM 37022T).
期刊介绍：
Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names.
The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including:
Identification, characterisation and culture preservation
Microbial evolution and biodiversity
Molecular environmental work with strong taxonomic or evolutionary content
Nomenclature
Taxonomy and phylogenetics.
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