{"title":"免疫组织化学检测her2阳性和模棱两可乳腺癌的基因改变。","authors":"Yi-Fang Tsai, Chih-Yi Hsu, Yun-Ning Chiu, Chi-Cheng Huang, Shih-Hsiang Chou, Yen-Shu Lin, Ta-Chung Chao, Chun-Yu Liu, Jen-Hwey Chiu, Ling-Ming Tseng","doi":"10.2147/BCTT.S507189","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>We aimed to identify genetic alterations in groups with different HER2 immunohistochemical (IHC) scores.</p><p><strong>Patients and methods: </strong>A total of 120 patients with HER2-positive breast cancers, including 89 cases with IHC 3+ tumors and 31 cases with IHC 2+ and positive for in situ hybridization (ISH) were enrolled. Molecular profiles were determined using Thermo Fisher TMO comprehensive assay on surgically removed tissues. All called variants were compared between IHC3+ and IHC2+/ISH+ groups by Fisher exact test.</p><p><strong>Results: </strong>There was a significantly higher sample frequency 94.4% (84/89) of <i>ERBB2</i> amplification in IHC3+ group than that in IHC2+/ISH+ group 45.2% (14/31). By contrast, there was a significantly lower sample frequency of <i>MYC</i>_AMP_CNA 10.1% (9/89) and <i>CCND3</i>_AMP_CNA 0% (0/89) in IHC3+ group than those in IHC2+/ISH+ group with sample frequency 25.8% (8/31), and 9.7% (3/31), respectively.</p><p><strong>Conclusion: </strong>We conclude that HER2 IHC3+ tumors have higher frequency of <i>ERBB2</i>_ AMP_CNA and lower frequency of <i>CCND3</i>_ AMP_CNA and <i>MYC</i>_AMP_CNA than IHC2+/ISH+ tumors. These results provide therapeutic strategies in treatment of HER2-positive breast cancer.</p>","PeriodicalId":9106,"journal":{"name":"Breast Cancer : Targets and Therapy","volume":"17 ","pages":"253-263"},"PeriodicalIF":3.3000,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11911235/pdf/","citationCount":"0","resultStr":"{\"title\":\"Genetic Alterations in HER2-Positive and Equivocal Breast Cancer by Immunohistochemistry.\",\"authors\":\"Yi-Fang Tsai, Chih-Yi Hsu, Yun-Ning Chiu, Chi-Cheng Huang, Shih-Hsiang Chou, Yen-Shu Lin, Ta-Chung Chao, Chun-Yu Liu, Jen-Hwey Chiu, Ling-Ming Tseng\",\"doi\":\"10.2147/BCTT.S507189\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>We aimed to identify genetic alterations in groups with different HER2 immunohistochemical (IHC) scores.</p><p><strong>Patients and methods: </strong>A total of 120 patients with HER2-positive breast cancers, including 89 cases with IHC 3+ tumors and 31 cases with IHC 2+ and positive for in situ hybridization (ISH) were enrolled. Molecular profiles were determined using Thermo Fisher TMO comprehensive assay on surgically removed tissues. All called variants were compared between IHC3+ and IHC2+/ISH+ groups by Fisher exact test.</p><p><strong>Results: </strong>There was a significantly higher sample frequency 94.4% (84/89) of <i>ERBB2</i> amplification in IHC3+ group than that in IHC2+/ISH+ group 45.2% (14/31). By contrast, there was a significantly lower sample frequency of <i>MYC</i>_AMP_CNA 10.1% (9/89) and <i>CCND3</i>_AMP_CNA 0% (0/89) in IHC3+ group than those in IHC2+/ISH+ group with sample frequency 25.8% (8/31), and 9.7% (3/31), respectively.</p><p><strong>Conclusion: </strong>We conclude that HER2 IHC3+ tumors have higher frequency of <i>ERBB2</i>_ AMP_CNA and lower frequency of <i>CCND3</i>_ AMP_CNA and <i>MYC</i>_AMP_CNA than IHC2+/ISH+ tumors. These results provide therapeutic strategies in treatment of HER2-positive breast cancer.</p>\",\"PeriodicalId\":9106,\"journal\":{\"name\":\"Breast Cancer : Targets and Therapy\",\"volume\":\"17 \",\"pages\":\"253-263\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2025-03-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11911235/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Breast Cancer : Targets and Therapy\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.2147/BCTT.S507189\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"ONCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Breast Cancer : Targets and Therapy","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2147/BCTT.S507189","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"ONCOLOGY","Score":null,"Total":0}
Genetic Alterations in HER2-Positive and Equivocal Breast Cancer by Immunohistochemistry.
Purpose: We aimed to identify genetic alterations in groups with different HER2 immunohistochemical (IHC) scores.
Patients and methods: A total of 120 patients with HER2-positive breast cancers, including 89 cases with IHC 3+ tumors and 31 cases with IHC 2+ and positive for in situ hybridization (ISH) were enrolled. Molecular profiles were determined using Thermo Fisher TMO comprehensive assay on surgically removed tissues. All called variants were compared between IHC3+ and IHC2+/ISH+ groups by Fisher exact test.
Results: There was a significantly higher sample frequency 94.4% (84/89) of ERBB2 amplification in IHC3+ group than that in IHC2+/ISH+ group 45.2% (14/31). By contrast, there was a significantly lower sample frequency of MYC_AMP_CNA 10.1% (9/89) and CCND3_AMP_CNA 0% (0/89) in IHC3+ group than those in IHC2+/ISH+ group with sample frequency 25.8% (8/31), and 9.7% (3/31), respectively.
Conclusion: We conclude that HER2 IHC3+ tumors have higher frequency of ERBB2_ AMP_CNA and lower frequency of CCND3_ AMP_CNA and MYC_AMP_CNA than IHC2+/ISH+ tumors. These results provide therapeutic strategies in treatment of HER2-positive breast cancer.
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