DNAzyme@MOF突破pH值限制检测间质液中的多巴胺。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-03-17 DOI:10.1016/j.bios.2025.117367

Jiuhong Zhao, Jinnan Zhao, Xiaoyu Zhang, Guixia Ling, Peng Zhang

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引用次数: 0

摘要

人体内多巴胺（DA）水平与神经系统疾病有一定的相关性。然而，大多数数据分析的检测方法都是复杂和昂贵的。本研究成功制备了pH稳定性提高的漆酶样DNAzyme@MOF。DNAzyme@MOF可以催化显色底物改变ISF中DA检测溶液的颜色。DNAzyme的加入使DNAzyme@MOF具有更高的稳定性和酶样活性。操作过程简单、快速、直观。此外，采用离线法分析了体内皮肤间质液（ISF）中DA的含量。制备肿胀水凝胶微针（MNs）提取皮肤ISF。漆酶样DNAzyme@MOF法检测皮肤ISF中DA的含量。本研究实现了DA的微创检测。本文提出的基于DNAzyme@MOF的ISF生物标志物检测方法将为未来ISF中其他生物标志物的检测提供一个新的发展方向。

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DNAzyme@MOF breaking pH limitation for the detection of dopamine in the interstitial fluid

The level of dopamine (DA) in the human body has a certain correlation with neurological diseases. However, most detection methods of DA are complex and expensive. In this study, laccase-like DNAzyme@MOF with improved pH stability was successfully prepared. DNAzyme@MOF could catalyze the chromogenic substrate to change the color of the solution for the detection of DA in ISF. The addition of DNAzyme made DNAzyme@MOF possess higher stability and enzyme-like activity. The operation process was simple, rapid, and intuitive. In addition, the in vivo DA content in skin interstitial fluid (ISF) was analyzed by an off-line method. The swelling hydrogel microneedles (MNs) were prepared to extract skin ISF. DA in skin ISF was recovered and detected by laccase-like DNAzyme@MOF. This study realized the minimally invasive detection of DA. The proposed detection method of biomarkers in ISF based on DNAzyme@MOF would provide a new dimension towards the future development for the detection of other biomarkers in ISF.

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.