Serum antibody titers against distemper, parvovirus and infectious hepatitis in dogs from Central Spain

A canine population's immune resistance to canine distemper virus (CDV), canine parvovirus (CPV), and infectious hepatitis virus (CAV-1) was evaluated. In this study, a total of 112 sera were analyzed. Animals were considered as vaccinated if, in the last two years, they had received at least one dose of a vaccine that provides joint protection against CDV, CPV, and CAV-1. Animals that had never received any dose of these vaccines were designated as non-vaccinated. CDV, CPV, and CAV-1 antibodies were detected via a modified solid-phase enzyme-linked immunosorbent assay (ELISA), which detects IgG antibody levels in sera and provides semi-quantitative results in <30 min. In total, 41.1 % of the dogs had been vaccinated, and 58.9 % of dogs were designated as non-vaccinated. Overall, 90.2 %, 92.0 %, and 78.6 % of the tested dogs had positive results for the presence of IgG antibodies against CPV, CDV, and CAV-1, respectively. CPV antibodies were present in 87.9 % (58/66) of the vaccinated and 93.5 % (43/46) of the non-vaccinated dogs, while CDV antibodies were present in 95.5 % (63/66) of the vaccinated and 87.0 % (40/46) of the non-vaccinated dogs. Finally, CAV-1 antibodies were present in 84.8 % (56/66) of the vaccinated and of 69.6 % (32/46) the non-vaccinated dogs.