一种抗菌、抗氧化的黏附海绵,通过没食子酸介导的纤维粘土在胶原蛋白中的坚固组装来控制动脉出血

IF 8.2 2区 材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY
Xin Guo, Xingling Zeng, Yeqing He, Xiaoxia Zhang, Wenli Shi, Ruimin Ran and Guoying Li*, 
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引用次数: 0

摘要

战场、临床手术和重大事故中的急性出血死亡是一个世界性的普遍问题。粘土基止血材料因其低成本和可靠的凝血活性而受到相当多的关注,特别是在严重出血的情况下,例如QuikClot,这是一种基于高岭土的止血纱布,是战场复苏的首选。然而,粘土颗粒的易脱离和相关的血栓形成风险严重阻碍了粘土基止血材料的发展。在这里,廉价的坡高岭土(Pal)纳米粘土被整合到胶原(COL)基质中,通过在粘土中加载Ca2+,并进一步使用没食子酸(GA)来介导粘土在COL基质上的强大组装。这种有针对性的界面设计是一种简单温和的方法,有效地改善了Pal颗粒的分散,降低了脱落的风险。与QuikClot超声洗涤2 min后水溶液明显浑浊不同,复合海绵(Ca-Pal-GA-COL)在超声洗涤10 min后水溶液保持清澈,并伴有82.71%的质量残留。复合海绵对大肠杆菌的抑制率为87.93%,具有良好的抗菌、抗氧化和组织粘附性能。重要的是,在混合模式下,Ca-Pal-GA-COL海绵在大鼠股动脉出血模型中的失血量(632 mg)和止血时间(151 s)比医用纱布(3850 mg和299 s)、纯COL海绵(1627 mg和201 s)和Pal-COL海绵(1494 mg和193 s)要少,与QuikClot (559 mg和142 s)相当。某些组织粘附特性使得Ca-Pal-GA-COL海绵比QuikClot更适合于严重的股动脉活动性出血情况。细胞实验证实该复合敷料具有一定的生物安全性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

An Antibacterial, Antioxidant Adherent Sponge Constructed for Control of Arterial Bleeding Via Gallic Acid-Mediated Robust Assembly of Fibrous Clay in Collagen

An Antibacterial, Antioxidant Adherent Sponge Constructed for Control of Arterial Bleeding Via Gallic Acid-Mediated Robust Assembly of Fibrous Clay in Collagen

Acute hemorrhage death on battlefields, during clinical surgeries, and in major accidents is a widespread worldwide problem. Clay-based hemostatic materials have received considerable attention for their low cost and reliable clotting activity, especially in cases of severe bleeding, such as QuikClot, which is a kaolin-based hemostatic gauze that is preferred for battlefield resuscitation. However, the easy detachment of clay particles and the associated risk of thrombosis have seriously hindered the development of clay-based hemostatic materials. Here, inexpensive palygorskite (Pal) nanoclay was integrated into the collagen (COL) matrix by loading Ca2+ in the clay and further using gallic acid (GA) to mediate the robust assembly of clay on the COL matrix. This targeted interfacial design is a simple and gentle method that effectively improves the dispersion of the Pal particles and reduces the risk of shedding. Unlike QuikClot where the aqueous solution was significantly turbid after 2 min of ultrasonic washing, the aqueous solution of the composite sponge (Ca-Pal-GA-COL) remained clear and was accompanied by 82.71% of the mass residue after 10 min of ultrasonic washing. The composite sponge also exhibited excellent antibacterial (87.93% inhibition rate of Escherichia coli), antioxidant, and tissue adhesion properties. Importantly, the Ca-Pal-GA-COL sponge exhibited less blood loss (632 mg) and a shorter hemostasis time (151 s) in a rat femoral artery hemorrhage model than the medical gauze (3850 mg and 299 s), pure COL sponge (1627 mg and 201 s), and Pal-COL sponge (1494 mg and 193 s) in a co-mingled mode, which are comparable to those of QuikClot (559 mg and 142 s). Furthermore, certain tissue adhesion properties render the Ca-Pal-GA-COL sponge more suitable than QuikClot for severe femoral artery active bleeding scenarios. Cellular experiments confirmed that the composite dressing has a certain biosafety.

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来源期刊
ACS Applied Materials & Interfaces
ACS Applied Materials & Interfaces 工程技术-材料科学:综合
CiteScore
16.00
自引率
6.30%
发文量
4978
审稿时长
1.8 months
期刊介绍: ACS Applied Materials & Interfaces is a leading interdisciplinary journal that brings together chemists, engineers, physicists, and biologists to explore the development and utilization of newly-discovered materials and interfacial processes for specific applications. Our journal has experienced remarkable growth since its establishment in 2009, both in terms of the number of articles published and the impact of the research showcased. We are proud to foster a truly global community, with the majority of published articles originating from outside the United States, reflecting the rapid growth of applied research worldwide.
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