细胞外囊泡制剂的定量脂质分析：展望

IF 15.5 1区医学 Q1 CELL BIOLOGY

Journal of Extracellular Vesicles Pub Date : 2025-03-16 DOI:10.1002/jev2.70049

Tore Skotland, Kim Ekroos, Alicia Llorente, Kirsten Sandvig

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引用次数: 0

摘要

质谱法进行定量脂质组学分析是测定细胞外囊泡（ev）脂质含量的必要条件。这种方法可以提供有关脂质总量、脂质种类组成、EV样品纯度以及EV细胞起源的信息。然而，在进行脂质分析时存在许多陷阱。因此，任何非专业人士都应该与脂质组学专家合作。除了许多关于脂质分析的好的评论文章外，我们推荐由国际脂质组学学会下属的兴趣小组脂质组学标准倡议组织发布的信息和指南。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Quantitative Lipid Analysis of Extracellular Vesicle Preparations: A Perspective

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Quantitative Lipid Analysis of Extracellular Vesicle Preparations: A Perspective

Quantitative lipidomic analysis performed by mass spectrometry is required for determination of the lipid content of extracellular vesicles (EVs). Such methods can provide information about the total amount of lipids, the lipid species composition, the purity of EV samples as well as the cellular origin of the EVs. There are, however, many pitfalls when performing lipid analyses. Thus, any non-specialist should collaborate with experts in lipidomics. In addition to many good review articles giving advice about lipid analyses, we recommend the information and guidelines published by the Lipidomic Standard Initiative, an interest group affiliated with the International Lipidomics Society.

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来源期刊

Journal of Extracellular Vesicles Biochemistry, Genetics and Molecular Biology-Cell Biology

CiteScore

27.30

自引率

4.40%

发文量

115

审稿时长

12 weeks

期刊介绍： The Journal of Extracellular Vesicles is an open access research publication that focuses on extracellular vesicles, including microvesicles, exosomes, ectosomes, and apoptotic bodies. It serves as the official journal of the International Society for Extracellular Vesicles and aims to facilitate the exchange of data, ideas, and information pertaining to the chemistry, biology, and applications of extracellular vesicles. The journal covers various aspects such as the cellular and molecular mechanisms of extracellular vesicles biogenesis, technological advancements in their isolation, quantification, and characterization, the role and function of extracellular vesicles in biology, stem cell-derived extracellular vesicles and their biology, as well as the application of extracellular vesicles for pharmacological, immunological, or genetic therapies. The Journal of Extracellular Vesicles is widely recognized and indexed by numerous services, including Biological Abstracts, BIOSIS Previews, Chemical Abstracts Service (CAS), Current Contents/Life Sciences, Directory of Open Access Journals (DOAJ), Journal Citation Reports/Science Edition, Google Scholar, ProQuest Natural Science Collection, ProQuest SciTech Collection, SciTech Premium Collection, PubMed Central/PubMed, Science Citation Index Expanded, ScienceOpen, and Scopus.