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IF 1.8 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Biomedical Chromatography Pub Date : 2025-03-17 DOI:10.1002/bmc.70059

Yang Liu, Yan Yang, Xing Wang, Shengyu Ge, Lele Li, Bo Feng, Lili Jin, Jiao Guan, Heyun Zhu

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引用次数: 0

摘要

黄芪桂枝五味煎剂（HGWD）是中药名方，在中国被广泛用于治疗糖尿病周围神经病变和硬皮病。考虑到黄芪桂枝五味煎剂不易保存和携带，我们的研究小组早期将其制成了黄芪桂枝五味颗粒。然而，HGWG 的体内代谢情况仍不清楚。本研究建立了超高效液相色谱-四极杆飞行时间质谱（UHPLC-Q-TOF-MS）方法，以鉴定大鼠口服桂枝五味颗粒后尿液和粪便中的原型和代谢物。在尿液和粪便样本中共鉴定出 100 种化合物。在尿液样本中，鉴定出 28 种原型化合物和 41 种相关代谢物。在粪便样本中，鉴定出 39 种原型化合物和 12 种相关代谢物。大鼠尿液和粪便中的主要原型化合物是单萜糖苷、类黄酮、有机酸、姜酚和萜烯。大鼠尿液中的主要代谢物是黄酮类相关代谢物、有机酸相关代谢物和姜酚相关代谢物，大鼠粪便中的主要代谢物是黄酮类相关代谢物。这项研究可为HGWG的药物代谢和新药开发研究提供理论依据。

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Metabolite Identification of Huangqi Guizhi Wuwu Granules in Rat Urine and Feces Based on Ultra–High-Performance Liquid Chromatography With Quadrupole Time-of-Flight Mass Spectrometry

Huangqi Guizhi Wuwu Decoction (HGWD), a famous Chinese medicine prescription, has been widely used in the treatment of diabetic peripheral neuropathy and scleroderma in China. Considering that the decoction was not easy to store and carry, our research group has converted HGWD into Huangqi Guizhi Wuwu Granules (HGWG) in the early stage. However, the in vivo metabolism profile of HGWG was still unclear. In this study, an ultra–high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) method was established to identify prototypes and metabolites in urine and feces of rats after oral administration of HGWG. A total of 100 compounds were identified in urine and feces samples. In the urine samples, 28 prototype compounds and 41 related metabolites were identified. In the feces samples, 39 prototype compounds and 12 related metabolites were identified. Monoterpenoid glucosides, flavonoids, organic acids, gingerols, and terpenes were the main prototype compounds in both rat urine and feces. Flavonoid-related metabolites, organic acid-related metabolites, and gingerol-related metabolites were the major metabolites in rat urine, and flavonoid-related metabolites were the major metabolites in rat feces. This study can provide a theoretical basis for the drug metabolism and new drug development research of HGWG.

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来源期刊

Biomedical Chromatography 生物-分析化学

CiteScore

3.60

自引率

5.60%

发文量

268

审稿时长

2.3 months

期刊介绍： Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.