为改善手术操作而设计的磷酸三钙泡沫和膏状骨移植材料对绵羊肩胛骨模型骨生成的影响

IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL
Norbert Neckel, Jonas Wüster, Li Xiang-Tischhauser, Schugufa Mir, Doaa Adel-Khattab, Benedikt Stang, Andrea Kuhr, Dirk Barnewitz, Antje Genzel, Steffen Koerdt, Carsten Rendenbach, Max Heiland, Susanne Nahles, Michael Stiller, Christine Knabe
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引用次数: 0

摘要

具有复杂形态缺损的临界尺寸骨缺损(CSDs)的重建在临床上仍然具有挑战性。为了避免自体骨移植的收获,越来越多的人寻求合适的人工骨移植材料。具有生物活性的β-磷酸三钙颗粒(TCP-G)的临床成功率表明,这些材料已成为一种极好的替代移植物选择。为了提高手术处理性能,TCP-G已与天然聚合物相结合,用于制造膏状和泡沫状材料,当移植给定的骨缺损或用注射器将其部署时,可以很容易地将其塑造成任何所需的形状。本研究评估了TCP膏状(TCP- p)和TCP-泡沫(TCP- f)植骨材料与TCP- g在羊肩胛骨CSD植入1、3、6、12和18个月后对骨形成和成骨标志物表达的影响,并验证了添加天然聚合物不会降低TCP- p和TCP-f的成骨性能的假设。为了量化骨形成和骨移植材料的吸收,采用组织形态学方法测定骨和骨移植材料的面积分数。在树脂包埋切片上对骨组织的各种细胞和基质成分中的I型胶原、骨钙素和骨涎蛋白表达进行免疫组化分析,以表征测试材料的成骨和生物活性特性。6个月时,所有三种TCP材料均促进了良好的缺损再生,并在12和18个月时进一步进行骨重塑。TCP-F和TCP-P分别在1个月和6个月时诱导了更大的骨钙素表达,并表现出更先进的移植物材料吸收。18个月时,所有三种植骨材料几乎完全被吸收,原始骨结构得以恢复。综上所述,TCP-P中的透明质酸和甲基纤维素成分以及TCP-F中的猪胶原成分并没有降低TCP-P和TCP-F的成骨能力,反而表现出更高的可吸收性和对成骨细胞OC表达的增强作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Effect of Tricalcium Phosphate Foam and Paste Bone Grafting Materials Designed for Improved Surgical Handling on Osteogenesis in a Sheep Scapula Model

Effect of Tricalcium Phosphate Foam and Paste Bone Grafting Materials Designed for Improved Surgical Handling on Osteogenesis in a Sheep Scapula Model

Reconstruction of critical-size bone defects (CSDs) with complex defect morphologies remains clinically challenging. The desire to avoid autograft harvesting has prompted an increasing quest for adequate synthetic bone grafting materials. The clinical success rates, which have been achieved with bioactive β-tricalcium phosphate granules (TCP-G) demonstrate that these materials have become an excellent alternative graft choice. In order to improve surgical handling properties, TCP-G have been combined with natural polymers for creating paste- and foam-like materials, which can easily be molded into any desired shape when grafting a given bony defect or deploying them with a syringe. This study assessed the effect of a TCP paste (TCP-P) and a TCP-foam (TCP-F) bone grafting material as compared to TCP-G on bone formation and osteogenic marker expression after 1, 3, 6, 12, and 18 months of implantation in CSD in the sheep scapula and tested the hypothesis that the addition of natural polymers would not diminish the osteogenic properties of TCP-P and TCP-F. The bone and bone graft material area fractions were determined histomorphometrically in order to quantify bone formation and bone graft material resorption. Immunohistochemical analysis of collagen type I, osteocalcin, and bone sialoprotein expression in the various cell and matrix components of the bone tissue was performed on resin-embedded sections for characterizing the osteogenic and bioactive properties of the test materials. By 6 months, all three TCP materials facilitated excellent defect regeneration with further bone remodeling at 12 and 18 months. TCP-F and TCP-P induced greater osteocalcin expression and exhibited more advanced graft material resorption at 1 and 6 months, respectively. At 18 months, all three grafting materials were almost fully resorbed with the original bony architecture being restored. Taken together, the hyaluronic acid and methylcellulose components in TCP-P and porcine collagen components in TCP-F did not diminish the osteogenic capacity of TCP-P and TCP-F, which exhibited an even slightly higher resorbability and enhancement effect on OC expression by osteoblasts.

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来源期刊
CiteScore
7.50
自引率
2.90%
发文量
199
审稿时长
12 months
期刊介绍: Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats: • original research reports • short research and development reports • scientific reviews • current concepts articles • special reports • editorials Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.
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