一种用于抗癌芯片应用的注射成型改性硅橡胶

IF 6.4 3区 材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY
Ben J. Haspels, Frederikus Bakker, Lucas J. A. M. Beckers, Roland Kanaar, Koen C. Kriege, Susanne M. Valster, Roland C. M. Vulders, Maayke M. P. Kuijten
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引用次数: 0

摘要

对于药物筛选和个性化医疗方法,芯片上的癌症(CoC)模型是有价值的,因为这些系统能够精确控制细胞和组织结构,并且可以配备微传感器来实时监测对药物的生理反应。微流控装置的精确设计对于再现肿瘤及其微环境的关键因素至关重要。聚二甲基硅氧烷(PDMS)由于其低成本、适合快速成型、光学透明性和生物相容性等优点而被广泛用作微流体材料。然而,PDMS的疏水性使其在微流体器件中的应用复杂化。本文描述了一种用于商用Micronit系统的注塑成型功能化PDMS流体插入件的特性和应用。注射成型的PDMS已经用羧基进行了修饰,以适应其表面的细胞培养,并以共价结合蛋白质,使PDMS表面持久涂层所需的流动条件。对PDMS进行改性不会改变其光学透明度高、不存在自荧光等优良性质。改进的PDMS流体插入适合在芯片上建立相关的癌症模型,并且不会显示小分子(如阿霉素)的吸收,允许它们用于芯片上的药物筛选和个性化医疗方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

An Injection-Molded Modified Silicone Rubber for Cancer-on-Chip Applications

An Injection-Molded Modified Silicone Rubber for Cancer-on-Chip Applications

For drug screening and personalized medicine approaches, cancer-on-chip (CoC) models are valuable as these systems enable precise control of cellular and tissue architecture and can be equipped with micro-sensors for real-time monitoring of physiological responses to drugs. Accurate design of the microfluidic device is important to allow recapitulation of key factors of a tumor and its microenvironment. Polydimethylsiloxane (PDMS) is widely used as material for microfluidics due to its favorable characteristics including low cost, suitability for rapid prototyping, optical transparency, and biocompatibility. However, the hydrophobic nature of PDMS complicates its use in microfluidic devices. Here, the characterization and application of an injection-molded functionalized PDMS fluidic insert for the commercially available Micronit system are described. The injection-molded PDMS has been modified with carboxyl groups to accommodate cell culture on its surface and to covalently bind proteins for long-lasting coating of the PDMS surface required for flow-conditions. Modification of PDMS does not change its favorable properties such as its high optical transparency and absence of auto-fluorescence. The modified PDMS fluidic inserts are suitable for building relevant cancer models on chip and do not show absorption of small molecules such as doxorubicin, allowing their use for drug screening and personalized medicine approaches on chip.

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来源期刊
Advanced Materials Technologies
Advanced Materials Technologies Materials Science-General Materials Science
CiteScore
10.20
自引率
4.40%
发文量
566
期刊介绍: Advanced Materials Technologies Advanced Materials Technologies is the new home for all technology-related materials applications research, with particular focus on advanced device design, fabrication and integration, as well as new technologies based on novel materials. It bridges the gap between fundamental laboratory research and industry.
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