黄粉虫蛋白水解物的制备及性质研究

IF 2.5 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY
Shengru Yang, Yinchen Hou, Qiushuang Zhang, Jian Zou, Jianlai Guo, Mengxing Lu, Wenhui Xiao, Kaige Wu, Tanchu Han, Wenfei Niu
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引用次数: 0

摘要

酶水解的蛋白质具有生物活性。以富含蛋白质、脱脂的黄粉虫为原料,经碱性蛋白酶水解。以水解度(DH)为指标,考察了温度、时间、pH、料液比、酶添加量等因素的影响;采用单向实验和响应面实验对酶解工艺进行优化,并对酶解产物的抗氧化性、理化性质和氨基酸组成进行分析。结果表明,制备酶解物的最佳工艺条件为:52℃,120 min, pH 7.5,酶投加量5000 U/g,料液比30:1,DH为13.82%。抗氧化分析表明,水解产物具有清除自由基的能力。当DPPH·浓度为0.4 mg/mL时,其清除率达47.51%,IC50为427.51 mg/mL。当其浓度为0.2 ~ 0.6 mg/mL时,OH·的清除率由50.41%提高到92.17%,IC50为158.4 mg/mL。水解产物的物理化学性质不同于黄粉虫蛋白。黄粉虫蛋白的溶解度在pH 11.0时达到最大值38.97%,而其水解产物在pH 5.0时溶解度达到最大值40.36%。pH为2.0和10.0时,水解产物的起泡率分别为26.58%和25.14%,起泡稳定性分别为61.43%和53.73%。水解产物的EAI和ESI在碱性条件下最佳,pH为10.0时最大,分别为0.82和44.5 m2/g。水解产物中含有多种氨基酸,芳香氨基酸占17.32%,疏水氨基酸占37.51%。水解产物中必需氨基酸占37.91%,水解产物EAAI值为0.7187;水解产物的蛋白质品质显著提高。水解产物具有较好的溶解度,可作为有效的抗氧化剂,为黄粉甲的开发利用提供理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Preparation and Properties of Tenebrio molitor Protein Hydrolysates

Preparation and Properties of Tenebrio molitor Protein Hydrolysates

Proteins hydrolyzed by enzymes were biologically active. The protein-rich, defatted Tenebrio molitor was used as raw material and hydrolyzed by alkaline protease. The effects of temperature, time, pH, liquid-to-feed ratio, and enzyme addition were investigated with degree of hydrolysis (DH) as an index; the enzymatic hydrolysis process was optimized using one-way and response surface experimental methods, and the antioxidant, physicochemical properties, and amino acid compositions of the hydrolysates were analyzed. The results showed that the optimum process conditions for the preparation of enzymatic hydrolysates were at 52°C for 120 min, pH 7.5, a dosage of enzyme of 5000 U/g, and a liquid-to-material ratio of 30:1, under which the DH was 13.82%. Antioxidant analysis revealed that the hydrolysates exhibited a scavenging ability for free radicals. The scavenging rate of DPPH· reached 47.51% when it was 0.4 mg/mL, and its IC50 was 427.51 mg/mL. When its concentration was 0.2–0.6 mg/mL, the scavenging rate of OH· increased from 50.41% to 92.17%, and its IC50 was 158.4 mg/mL. The physicochemical properties of the hydrolysates differed from those of the Tenebrio molitor protein. The solubility of Tenebrio molitor protein reached a maximum of 38.97% at pH 11.0, whereas that of its hydrolysates peaked at 40.36% at pH 5.0. The hydrolysates exhibited higher values at pH 2.0 and 10.0, with foaming capacities of 26.58% and 25.14% and foaming stabilities of 61.43% and 53.73%, respectively. The EAI and ESI of the hydrolysates were optimal under alkaline conditions, with maximum values of 0.82 and 44.5 m2/g, respectively, at pH 10.0. The hydrolysates contained a variety of amino acids, as well as aromatic amino acids (17.32%) and hydrophobic amino acids (37.51%). Essential amino acids in hydrolysates accounted for 37.91%, and the EAAI value of hydrolysates was 0.7187; the protein quality of hydrolysates significantly improved. The hydrolysates had better solubility and can be used as an effective antioxidant, and the study could provide a theoretical basis for the development and utilization of Tenebrio molitor.

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来源期刊
CiteScore
5.30
自引率
12.00%
发文量
1000
审稿时长
2.3 months
期刊介绍: The journal presents readers with the latest research, knowledge, emerging technologies, and advances in food processing and preservation. Encompassing chemical, physical, quality, and engineering properties of food materials, the Journal of Food Processing and Preservation provides a balance between fundamental chemistry and engineering principles and applicable food processing and preservation technologies. This is the only journal dedicated to publishing both fundamental and applied research relating to food processing and preservation, benefiting the research, commercial, and industrial communities. It publishes research articles directed at the safe preservation and successful consumer acceptance of unique, innovative, non-traditional international or domestic foods. In addition, the journal features important discussions of current economic and regulatory policies and their effects on the safe and quality processing and preservation of a wide array of foods.
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