极化羟基磷灰石-海藻酸钠复合材料作为抗菌填料基质

IF 1.8 4区 生物学 Q3 BIOPHYSICS
Samapika Bhuyan, Subhasmita Swain, Tapash Ranjan Rautray
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引用次数: 0

摘要

基于生物陶瓷和聚合物的骨替代复合材料具有生物活性、生物相容性、骨整合性和机械稳定性等动态特性,可用于控制药物输送系统,以避免感染和疼痛。在这项研究中,我们开发了一种通过表面极化在生物材料基质上诱导抗菌和成骨反应的新方法。羟基磷灰石-海藻酸钠复合材料使用电晕极化装置进行负极化,并使用x射线衍射分析进行表征。热激去极化电流研究表明,在480℃温度下,最大电流为4.74 nA/cm2。用接触角测量了试样的润湿性,结果表明极化复合试样具有较高的保水能力。细菌细胞活力测试使用3-(4,5 -二甲基噻唑-2)- 2,5 -二苯基溴化四唑)测定法进行测量,结果显示,与未极化的样品相比,极化样品上的细菌生长较差。此外,在极化标本上,成骨MG63细胞增殖表现出基因表达增加。结果表明,极化羟基磷灰石-海藻酸钠复合材料具有抗菌和成骨活性,可作为一种较好的抗菌骨填充基质。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Polarised hydroxyapatite- sodium alginate composite as an antibacterial filler matrix

Polarised hydroxyapatite- sodium alginate composite as an antibacterial filler matrix

Bone-substituted composite material based on bioceramics and polymer has enhanced their biological performance with dynamic properties such as bioactivity, biocompatibility, osseointegration, and mechanical stability, which can be used in a controlled drug delivery system for avoiding infections as well as pain. Here in this study, we developed a new approach for inducing antibacterial and osteogenic responses on biomaterial substrates via surface polarisation. The hydroxyapatite- sodium alginate composite was negatively polarised using a corona poling setup and characterised using X-ray diffraction analysis. The thermally stimulated depolarization current study showed a maximum current of 4.74 nA/cm2, observed at a temperature of 480 °C. The wettability of the specimen was measured using contact angle measurements, which demonstrated that the polarised composite specimen exhibited higher water retention ability. The bacterial cell viability test was measured using the 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay, which revealed poor bacterial growth on polarised specimens as compared to their unpolarised counterparts. In addition, the osteogenic MG63 cell proliferation showed increased gene expression on polarised specimens. These findings showed that polarising hydroxyapatite- sodium alginate composite could be an excellent option to be used as an antibacterial bone filler matrix for faster healing as it showed both antibacterial and osteogenic activity.

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来源期刊
Journal of Biological Physics
Journal of Biological Physics 生物-生物物理
CiteScore
3.00
自引率
5.60%
发文量
20
审稿时长
>12 weeks
期刊介绍: Many physicists are turning their attention to domains that were not traditionally part of physics and are applying the sophisticated tools of theoretical, computational and experimental physics to investigate biological processes, systems and materials. The Journal of Biological Physics provides a medium where this growing community of scientists can publish its results and discuss its aims and methods. It welcomes papers which use the tools of physics in an innovative way to study biological problems, as well as research aimed at providing a better understanding of the physical principles underlying biological processes.
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