糖修饰的胆固醇核心纳米颗粒作为潜在的靶向纳米药物递送亲脂性药物

Laís Rossetto Ferraz de Barros, Carlos Eduardo de Castro, Anabella Patricia Rosso, Rodrigo da Costa Duarte, Alexandre Gonçalves Dal-Bó, Wendel Andrade Alves and Fernando Carlos Giacomelli
{"title":"糖修饰的胆固醇核心纳米颗粒作为潜在的靶向纳米药物递送亲脂性药物","authors":"Laís Rossetto Ferraz de Barros, Carlos Eduardo de Castro, Anabella Patricia Rosso, Rodrigo da Costa Duarte, Alexandre Gonçalves Dal-Bó, Wendel Andrade Alves and Fernando Carlos Giacomelli","doi":"10.1039/D4PM00317A","DOIUrl":null,"url":null,"abstract":"<p >Targeted drug delivery is a precise and effective strategy in oncotherapy and can be achieved through sugar-decorated assemblies since glucose receptors are overexpressed on cancer cell membranes to compensate for their increased glucose demands. In this study, core–shell nanoparticles (NPs) were synthesized using amphiphilic macromolecules comprising hydrophobic cholesterol (Chol) segments conjugated to hydrophilic polyethylene oxide containing azide group (Chol-PEO<small><sub>22</sub></small>-N<small><sub>3</sub></small>) or substituted with the carbohydrate <em>N</em>-acetyl-<small>D</small>-glucosamine (Chol-PEO<small><sub>22</sub></small>-GlcNAc) <em>via</em> a click chemistry reaction. These self-assemblies, which are smaller than 100 nm and suitable for cancer treatment, demonstrated efficient loading efficiency (exceeding 70%) with ursolic acid (UA), a hydrophobic drug, serving as a proof-of-concept for targeted therapy using natural compounds against non-small cell lung cancer. The incorporation of sugar molecules modified the structural characteristics of the nanocarriers, resulting in larger and presumably less dense particles. This modification influenced the UA release mechanism, leading to a faster and nearly complete release over a week, whereas approximately 60% of the encapsulated UA remained entrapped in the Chol-PEO<small><sub>22</sub></small>-N<small><sub>3</sub></small> NPs. Enhanced cell cytotoxicity was achieved with UA-loaded NPs with <em>in vitro</em> cell viability assays indicating at least two-fold increase in the inhibitory effect of the drug-loaded nanocarriers. The targeted delivery was also demonstrated as UA-loaded Chol-PEO<small><sub>22</sub></small>-GlcNAc NPs showed greater internalization by cancer cells than their healthy counterparts.</p>","PeriodicalId":101141,"journal":{"name":"RSC Pharmaceutics","volume":" 2","pages":" 387-397"},"PeriodicalIF":0.0000,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/pm/d4pm00317a?page=search","citationCount":"0","resultStr":"{\"title\":\"Sugar-decorated cholesterol-core nanoparticles as potential targeting nanomedicines for the delivery of lipophilic drugs†\",\"authors\":\"Laís Rossetto Ferraz de Barros, Carlos Eduardo de Castro, Anabella Patricia Rosso, Rodrigo da Costa Duarte, Alexandre Gonçalves Dal-Bó, Wendel Andrade Alves and Fernando Carlos Giacomelli\",\"doi\":\"10.1039/D4PM00317A\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Targeted drug delivery is a precise and effective strategy in oncotherapy and can be achieved through sugar-decorated assemblies since glucose receptors are overexpressed on cancer cell membranes to compensate for their increased glucose demands. In this study, core–shell nanoparticles (NPs) were synthesized using amphiphilic macromolecules comprising hydrophobic cholesterol (Chol) segments conjugated to hydrophilic polyethylene oxide containing azide group (Chol-PEO<small><sub>22</sub></small>-N<small><sub>3</sub></small>) or substituted with the carbohydrate <em>N</em>-acetyl-<small>D</small>-glucosamine (Chol-PEO<small><sub>22</sub></small>-GlcNAc) <em>via</em> a click chemistry reaction. These self-assemblies, which are smaller than 100 nm and suitable for cancer treatment, demonstrated efficient loading efficiency (exceeding 70%) with ursolic acid (UA), a hydrophobic drug, serving as a proof-of-concept for targeted therapy using natural compounds against non-small cell lung cancer. The incorporation of sugar molecules modified the structural characteristics of the nanocarriers, resulting in larger and presumably less dense particles. This modification influenced the UA release mechanism, leading to a faster and nearly complete release over a week, whereas approximately 60% of the encapsulated UA remained entrapped in the Chol-PEO<small><sub>22</sub></small>-N<small><sub>3</sub></small> NPs. Enhanced cell cytotoxicity was achieved with UA-loaded NPs with <em>in vitro</em> cell viability assays indicating at least two-fold increase in the inhibitory effect of the drug-loaded nanocarriers. The targeted delivery was also demonstrated as UA-loaded Chol-PEO<small><sub>22</sub></small>-GlcNAc NPs showed greater internalization by cancer cells than their healthy counterparts.</p>\",\"PeriodicalId\":101141,\"journal\":{\"name\":\"RSC Pharmaceutics\",\"volume\":\" 2\",\"pages\":\" 387-397\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-01-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://pubs.rsc.org/en/content/articlepdf/2025/pm/d4pm00317a?page=search\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"RSC Pharmaceutics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2025/pm/d4pm00317a\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"RSC Pharmaceutics","FirstCategoryId":"1085","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2025/pm/d4pm00317a","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

靶向药物递送是一种精确有效的肿瘤治疗策略,由于葡萄糖受体在癌细胞膜上过度表达以补偿其增加的葡萄糖需求,因此可以通过糖修饰组装来实现。本研究利用疏水胆固醇(Chol)片段与含叠氮化物基团的亲水聚氧化物(choll - peo22 - n3)或碳水化合物n -乙酰- d -氨基葡萄糖(choll - peo22 - glcnac)偶联的两亲性大分子合成了核壳纳米颗粒(NPs)。这些小于100纳米的自组装体适用于癌症治疗,显示出熊果酸(UA)(一种疏水药物)的高效负载效率(超过70%),作为使用天然化合物靶向治疗非小细胞肺癌的概念验证。糖分子的掺入改变了纳米载体的结构特征,导致颗粒更大,可能密度更低。这种修饰影响了UA的释放机制,导致在一周内更快且几乎完全释放,而大约60%的封装UA仍然被困在choll - peo22 - n3 NPs中。负载ua的NPs增强了细胞毒性,体外细胞活力测定表明,负载药物的纳米载体的抑制作用至少增加了两倍。靶向递送也被证明是ua负载的cholo - peo22 - glcnac NPs比其健康对应物更容易被癌细胞内化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Sugar-decorated cholesterol-core nanoparticles as potential targeting nanomedicines for the delivery of lipophilic drugs†

Sugar-decorated cholesterol-core nanoparticles as potential targeting nanomedicines for the delivery of lipophilic drugs†

Targeted drug delivery is a precise and effective strategy in oncotherapy and can be achieved through sugar-decorated assemblies since glucose receptors are overexpressed on cancer cell membranes to compensate for their increased glucose demands. In this study, core–shell nanoparticles (NPs) were synthesized using amphiphilic macromolecules comprising hydrophobic cholesterol (Chol) segments conjugated to hydrophilic polyethylene oxide containing azide group (Chol-PEO22-N3) or substituted with the carbohydrate N-acetyl-D-glucosamine (Chol-PEO22-GlcNAc) via a click chemistry reaction. These self-assemblies, which are smaller than 100 nm and suitable for cancer treatment, demonstrated efficient loading efficiency (exceeding 70%) with ursolic acid (UA), a hydrophobic drug, serving as a proof-of-concept for targeted therapy using natural compounds against non-small cell lung cancer. The incorporation of sugar molecules modified the structural characteristics of the nanocarriers, resulting in larger and presumably less dense particles. This modification influenced the UA release mechanism, leading to a faster and nearly complete release over a week, whereas approximately 60% of the encapsulated UA remained entrapped in the Chol-PEO22-N3 NPs. Enhanced cell cytotoxicity was achieved with UA-loaded NPs with in vitro cell viability assays indicating at least two-fold increase in the inhibitory effect of the drug-loaded nanocarriers. The targeted delivery was also demonstrated as UA-loaded Chol-PEO22-GlcNAc NPs showed greater internalization by cancer cells than their healthy counterparts.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信