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IF 4.5 3区工程技术 Q1 CHEMISTRY, APPLIED

Reactive & Functional Polymers Pub Date : 2025-03-07 DOI:10.1016/j.reactfunctpolym.2025.106229

Liying Peng , Li Chang , Rushui Bai , Qiannan Sun , Yunfan Zhang , Hongliang Liu , Chuao Ma , Jiuxiang Lin , Bing Han

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引用次数: 0

摘要

本研究利用表面引发的原子转移自由基聚合（SI-ATPR）将齐聚离子聚合物聚（羧基甜菜碱甲基丙烯酸酯）（PCBMA）接枝到氧化石墨烯（GO）（GO/PCBMA）上，从而增强其理化性质和生物医学潜力。X 射线光电子能谱（XPS）和傅立叶变换红外光谱（FTIR）证实了接枝的成功，而热重分析（TGA）表明 PCBMA 的负载量为 0.54 mg/mg GO。凝胶渗透色谱法（GPC）显示单体转换效率为 40%，表明聚合控制良好。GO/PCBMA 表现出卓越的抗菌性能，当浓度为 80 μg/mL 时，可在 5 小时内将变异链球菌的存活率降低 87.6%，而 GO 需要 160 μg/mL 才能将存活率降低 78.3%。三天的菌落形成单位（CFU）计数进一步验证了长期抗菌效果。GO/PCBMA 还提高了 L929 成纤维细胞的存活率，促进了人骨髓间充质干细胞（hBMMSCs）的成骨分化，碱性磷酸酶（ALP）活性和骨钙素（OCN）表达的增加证明了这一点。这些发现突出表明，GO/PCBMA 是一种很有前景的材料，可用于要求抗菌活性和生物相容性的应用领域，特别是长期生物医学植入物。这项研究为开发基于氧化石墨烯的多功能生物材料提供了启示。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Zwitterion polymer-modified graphene oxides enhance antibacterial activity with improved biocompatibility and osteogenesis: An in vitro study

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Zwitterion polymer-modified graphene oxides enhance antibacterial activity with improved biocompatibility and osteogenesis: An in vitro study

This study utilized surface-initiated atom transfer radical polymerization (SI-ATRP) to graft the zwitterionic polymer poly(carboxybetaine methacrylate) (PCBMA) onto graphene oxide (GO) (GO/PCBMA), enhancing its physicochemical properties and biomedical potential. X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR) confirmed successful grafting, while thermogravimetric analysis (TGA) indicated a PCBMA loading of 0.54 mg/mg GO. Gel permeation chromatography (GPC) showed a monomer conversion efficiency of ∼40 %, demonstrating good polymerization control. GO/PCBMA exhibited superior antibacterial performance, reducing Streptococcus mutans viability by 87.6 % at 80 μg/mL within 5 h, while GO required 160 μg/mL to achieve a 78.3 % reduction. Long-term antibacterial effects were further validated through three-day colony-forming unit (CFU) counts. GO/PCBMA also improved L929 fibroblast viability and promoted osteogenic differentiation in human bone marrow mesenchymal stem cells (hBMMSCs), as evidenced by increased alkaline phosphatase (ALP) activity and osteocalcin (OCN) expression. These findings highlight GO/PCBMA as a promising material for applications requiring both antibacterial activity and biocompatibility, particularly for long-term biomedical implants. This study provides insights for the development of multifunctional graphene oxide-based biomaterials.

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来源期刊

Reactive & Functional Polymers 工程技术-高分子科学

CiteScore

8.90

自引率

5.90%

发文量

259

审稿时长

27 days

期刊介绍： Reactive & Functional Polymers provides a forum to disseminate original ideas, concepts and developments in the science and technology of polymers with functional groups, which impart specific chemical reactivity or physical, chemical, structural, biological, and pharmacological functionality. The scope covers organic polymers, acting for instance as reagents, catalysts, templates, ion-exchangers, selective sorbents, chelating or antimicrobial agents, drug carriers, sensors, membranes, and hydrogels. This also includes reactive cross-linkable prepolymers and high-performance thermosetting polymers, natural or degradable polymers, conducting polymers, and porous polymers. Original research articles must contain thorough molecular and material characterization data on synthesis of the above polymers in combination with their applications. Applications include but are not limited to catalysis, water or effluent treatment, separations and recovery, electronics and information storage, energy conversion, encapsulation, or adhesion.