高灵敏度荧光探针，用于准确检测线粒体中的过氧化亚硝酸盐并为炎症部位成像

IF 4.1 3区工程技术 Q2 CHEMISTRY, APPLIED

Dyes and Pigments Pub Date : 2025-03-11 DOI:10.1016/j.dyepig.2025.112756

Xiao-Lei Xue , Ru-Xing Chen , Jun-Yu Luan , Ying-Ying Shan , Kun-Peng Wang , Xiao-Zhong Xiang , Ying Fang , Shaojin Chen , Zhi-Qiang Hu

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引用次数: 0

摘要

过氧化亚硝酸盐（ONOO-）在各种生理和病理过程中发挥着关键作用。灵敏而特异地检测 ONOO- 对疾病的早期诊断、治疗和预后具有重要意义。本研究开发了一种近红外（NIR）荧光探针，用于快速（小于 100 秒）检测 ONOO-。随着 ONOO- 浓度的增加，荧光强度比与 ONOO- 浓度呈稳健的线性关系。此外，该探针还能选择性地检测 ONOO- 水平的变化，检测限（LOD）值为 13 nM。重要的是，该探针被成功用于检测活细胞和小鼠模型中的内源性 ONOO-。总之，该荧光探针在阐明ONOO-诱导的疾病过程方面具有巨大潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Highly sensitive fluorescence probe for accurate detection of peroxynitrite in mitochondria and imaging inflammation sites

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Highly sensitive fluorescence probe for accurate detection of peroxynitrite in mitochondria and imaging inflammation sites

Peroxynitrite (ONOO⁻) plays a pivotal role in various physiological and pathological processes. The sensitive and specific detection of ONOO⁻ is of paramount significance for early diagnosis, treatment, and prognosis of diseases. In this study, a near-infrared (NIR) fluorescent probe was developed for the detection of ONOO⁻ with rapid responsiveness (less than 100 s). As the concentration of ONOO⁻ increases, the ratio of fluorescence intensities exhibited a robust linear relationship with ONOO⁻ concentration. Additionally, the probe demonstrated the ability to selectively detect changes in ONOO⁻ levels, with a limit of detection (LOD) value of 13 nM. Importantly, the probe was successfully employed to detect endogenous ONOO⁻ in living cells and mouse models. In conclusion, the fluorescent probe exhibits significant potential for elucidating disease processes induced by ONOO⁻.

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来源期刊

Dyes and Pigments 工程技术-材料科学：纺织

CiteScore

8.20

自引率

13.30%

发文量

933

审稿时长

33 days

期刊介绍： Dyes and Pigments covers the scientific and technical aspects of the chemistry and physics of dyes, pigments and their intermediates. Emphasis is placed on the properties of the colouring matters themselves rather than on their applications or the system in which they may be applied. Thus the journal accepts research and review papers on the synthesis of dyes, pigments and intermediates, their physical or chemical properties, e.g. spectroscopic, surface, solution or solid state characteristics, the physical aspects of their preparation, e.g. precipitation, nucleation and growth, crystal formation, liquid crystalline characteristics, their photochemical, ecological or biological properties and the relationship between colour and chemical constitution. However, papers are considered which deal with the more fundamental aspects of colourant application and of the interactions of colourants with substrates or media. The journal will interest a wide variety of workers in a range of disciplines whose work involves dyes, pigments and their intermediates, and provides a platform for investigators with common interests but diverse fields of activity such as cosmetics, reprographics, dye and pigment synthesis, medical research, polymers, etc.