用于快速和无标记蛋白质检测的自动电渗透数字光流体

IF 9.1 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Letters Pub Date : 2025-03-16 DOI:10.1021/acs.nanolett.5c00270

Fan Yang, Rongxin Fu, Yitong Liu, Wenbo Dong, Xuekai Liu, Yan Song, Gong Li, Tianqi Zhou, Hanqi Hu, Shanglin Li, Xiangyu Jin, Jiangjiang Zhang, Hang Li, Yao Lu, Yanfang Guan, Tianming Xu, He Ding, Guoliang Huang, Huikai Xie, Shuailong Zhang

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引用次数: 0

摘要

快速蛋白质检测对于医学诊断、临床试验和药物开发至关重要，但在平衡多重检测、低试剂消耗和短检测时间的敏感性方面往往面临挑战。在这项工作中，我们开发了一个自动化和敏感的电渗透数字光流体（e-DOF）平台，用于快速和无标记地定量微升血液样本中的蛋白质生物标志物。高光谱计算揭示了靶蛋白捕获引起的纳米级形态变化，消除了多种酶联标记。电渗透驱动的分子循环加速了免疫杂交，提高了灵敏度（检测限为0.21 nM），并将检测时间缩短至15分钟，而传统的酶联免疫吸附测定则需要2-3小时。在17份临床样本中多重检测甲型和戊型肝炎IgM，结果与临床试验结果完全一致。该e-DOF系统为微升样品的多重检测提供了一个自动化，快速和无标签的平台，突出了在临床诊断和免疫分析研究中的潜在应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Automated Electroosmotic Digital Optofluidics for Rapid and Label-Free Protein Detection

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Automated Electroosmotic Digital Optofluidics for Rapid and Label-Free Protein Detection

Rapid protein detection is crucial for medical diagnosis, clinical trials, and drug development but often faces challenges in balancing sensitivity with multiplex detection, low reagent consumption, and a short detection time. In this work, we developed an automated and sensitive electroosmotic digital optofluidics (e-DOF) platform for rapid and label-free protein biomarker quantification in microliter blood samples. The hyperspectral computation reveals nanoscale morphology changes caused by target protein capture, eliminating multifarious enzyme-linked labeling. Electroosmosis-driven molecular circulation accelerates the immuno-hybridization, enhancing sensitivity (with a detection limit of 0.21 nM) and reducing the detection time to 15 min, compared to 2–3 h for a traditional enzyme-linked immunosorbent assay. In multiplex detection of hepatitis A and E IgM in 17 clinical samples, the results were completely consistent with clinical trial outcomes. This e-DOF system presents an automated, rapid, and label-free platform for multiplex detection in microliter samples, highlighting potential applications in clinical diagnosis and immunoassay research.

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来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.