小鼠颈动脉灌注原位脑组织固定及平行非固定组织收集方案。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2025-06-20 Epub Date: 2025-03-14 DOI:10.1016/j.xpro.2025.103699

Phung N Thai, Zubayer I Ferdous, Pauline Trinh, Valeriy Timofeev, Daphne A Diloretto, Ning Zong, Chao-Yin Chen, Nipavan Chiamvimonvat, Xiao-Dong Zhang

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引用次数: 0

摘要

随着对多器官功能中枢控制的研究日益突出，对同一实验动物固定脑和非固定器官组织的采集需求日益增加。在这里，我们提出了一种在小鼠中进行颈动脉插管、器官和组织收集、原位脑灌注和固定以及脑解剖的方案。我们描述了颈动脉插管，采集心脏和其他器官，灌注，固定和解剖大脑的步骤。

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Protocol for mouse carotid artery perfusion for in situ brain tissue fixation and parallel unfixed tissue collection.

As the study of central control of multiple organ function becomes more prominent, there is an increasing need for the collection of fixed brain and unfixed organs and tissues from the same experimental animal. Here, we present a protocol for performing carotid artery cannulation, organ and tissue collection, in situ brain perfusion and fixation, and brain dissection in mice. We describe steps for cannulating the carotid artery, harvesting the heart and other organs, and perfusing, fixing, and dissecting the brain.

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