Eric Whitters, Kelline Rodems, Yinglei Tao, Tom de Poorter
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Developing novel test for allergy diagnostics: using today’s biochemical techniques
Background
The key to moving beyond “bucket chemistry” in allergy testing is to tightly control the sourcing, analysis, and performance testing of the extracts used. It is imperative that each allergen extract be scrutinized using today’s biochemical techniques as an active part of the qualification process rather than the ones developed almost 30 years ago for the technologies of the 1990s. Characterization of allergens demystifies the source material and provides results in the clinical lab that are less ambiguous and are more closely related with the clinical status of the patient.
Methods and results
This article delivers a technical overview that outlines the requisite steps for diagnostic companies to characterize, analyze, and qualify allergen extracts, mixtures, and components integrated into their platforms. It also evaluates diverse technologies employed in both singleplex and multiplex diagnostics, highlighting the incorporation of more precisely defined biochemical materials in their product offerings.
Conclusion
The primary goal of this review is to provide laboratory professionals and clinicians with a systematic approach to ensure that extracts meet stringent performance requirements crucial for dependable allergen-specific IgE testing.
期刊介绍：
Allergo Journal International is the official Journal of the German Society for Applied Allergology (AeDA) and the Austrian Society for Allergology and Immunology (ÖGAI). The journal is a forum for the communication and exchange of ideas concerning the various aspects of allergy (including related fields such as clinical immunology and environmental medicine) and promotes German allergy research in an international context. The aim of Allergo Journal International is to provide state of the art information for all medical and scientific disciplines that deal with allergic, immunological and environmental diseases. Allergo Journal International publishes original articles, reviews, short communications, case reports, and letters to the editor. The articles cover topics such as allergic, immunological and environmental diseases, the latest developments in diagnosis and therapy as well as current research work concerning antigens and allergens and aspects related to occupational and environmental medicine. In addition, it publishes clinical guidelines and position papers approved by expert panels of the German, Austrian and Swiss Allergy Societies.
All submissions are reviewed in single-blind fashion by at least two reviewers.
Originally, the journal started as a German journal called Allergo Journal back in 1992. Throughout the years, English articles amounted to a considerable portion in Allergo Journal. This was one of the reasons to extract the scientific content and publish it in a separate journal. Hence, Allergo Journal International was born and now is the international continuation of the original German journal. Nowadays, all original content is published in Allergo Journal International first. Later, selected manuscripts will be translated and published in German and included in Allergo Journal.
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