发酵强化生物除磷过程中的高阶挥发性脂肪酸代谢和非典型多羟基烷酸生产

IF 11.4 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Water Research Pub Date : 2025-03-15 DOI:10.1016/j.watres.2025.123503

R. Thomson , C. Le , L. Wang , D.J. Batstone , Y. Zhou , A. Oehmen

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引用次数: 0

摘要

增强型生物除磷（EBPR）是一种成熟的废水处理工艺，但其广泛应用受到高温和低碳可用性等因素的限制。发酵增强EBPR （F-EBPR）工艺在解决这些限制方面显示出希望，但其潜在机制尚未完全了解。本研究利用碳同位素标记和散弹枪宏基因组测序分析相结合的方法研究了F-EBPR系统中高阶（C4-5）挥发性脂肪酸（VFAs）的代谢。结果表明，丁酸盐（HBu）的摄取通过β-氧化和标准缩合途径的结合导致典型（C4-5）和非典型（C6+）聚羟基烷酸盐（PHAs）的形成，而HBu氧化剂在F-EBPR过程中相对于底物组成确定了可能的作用。宏基因组分析显示，在聚磷酸盐积累生物（PAOs）和糖原积累生物（GAOs）中都存在高阶VFA代谢所需的基因。本研究还强调了当前模型在描述F-EBPR过程中的局限性，并强调需要改进模型来解释高阶VFA代谢和微生物群落动态。

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Higher order volatile fatty acid metabolism and atypical polyhydroxyalkanoate production in fermentation-enhanced biological phosphorus removal

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Higher order volatile fatty acid metabolism and atypical polyhydroxyalkanoate production in fermentation-enhanced biological phosphorus removal

Enhanced biological phosphorus removal (EBPR) is an established wastewater treatment process, but its wider implementation has been limited by factors like high temperature and low carbon availability. Fermentation-enhanced EBPR (F-EBPR) processes have shown promise in addressing these limitations, but the underlying mechanisms are not fully understood. This study investigates the metabolism of higher order (C_4–5) volatile fatty acids (VFAs) in F-EBPR systems using a combination of carbon isotope labelling and shotgun metagenomic sequencing analyses. Results show that butyrate (HBu) uptake leads to the formation of both typical (C_4–5) and atypical (C₆₊) polyhydroxyalkanoates (PHAs) through a combination ofβ-oxidation and standard condensation pathways, while the putative role of HBu oxidisers were identified relative to substrate composition in F-EBPR processes. Metagenomic analysis reveals the presence of genes required for higher order VFA metabolism in both polyphosphate accumulating organisms (PAOs) and glycogen accumulating organisms (GAOs). This study also highlights the limitations of current models in describing F-EBPR processes and emphasises the need for improved models that account for higher order VFA metabolism and microbial community dynamics.

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来源期刊

Water Research 环境科学-工程：环境

CiteScore

20.80

自引率

9.40%

发文量

1307

审稿时长

38 days

期刊介绍： Water Research, along with its open access companion journal Water Research X, serves as a platform for publishing original research papers covering various aspects of the science and technology related to the anthropogenic water cycle, water quality, and its management worldwide. The audience targeted by the journal comprises biologists, chemical engineers, chemists, civil engineers, environmental engineers, limnologists, and microbiologists. The scope of the journal include: •Treatment processes for water and wastewaters (municipal, agricultural, industrial, and on-site treatment), including resource recovery and residuals management; •Urban hydrology including sewer systems, stormwater management, and green infrastructure; •Drinking water treatment and distribution; •Potable and non-potable water reuse; •Sanitation, public health, and risk assessment; •Anaerobic digestion, solid and hazardous waste management, including source characterization and the effects and control of leachates and gaseous emissions; •Contaminants (chemical, microbial, anthropogenic particles such as nanoparticles or microplastics) and related water quality sensing, monitoring, fate, and assessment; •Anthropogenic impacts on inland, tidal, coastal and urban waters, focusing on surface and ground waters, and point and non-point sources of pollution; •Environmental restoration, linked to surface water, groundwater and groundwater remediation; •Analysis of the interfaces between sediments and water, and between water and atmosphere, focusing specifically on anthropogenic impacts; •Mathematical modelling, systems analysis, machine learning, and beneficial use of big data related to the anthropogenic water cycle; •Socio-economic, policy, and regulations studies.