低温心肌保存：冷冻辩论。

IF 0.8 4区医学 Q4 IMMUNOLOGY

Transplantation proceedings Pub Date : 2025-03-14 DOI:10.1016/j.transproceed.2025.02.035

Mohammed Quader , Zachary Kiernan , Gina Labate , Qun Chen

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引用次数: 0

摘要

背景：在冰中储存心脏用于运输被认为会导致继发于冻伤的移植后移植物功能障碍。我们研究了心脏在0°C、4°C和8°C下保存4小时的心肌损伤及其功能。方法：在全身麻醉下获取大鼠心脏，立即在威斯康星大学溶液中于0°C、4°C和8°C保存4小时（n = 6-8/组）。然后在Langendorff仪器上使用含氧Kreb-Henseleit缓冲液（37°C）再灌注90分钟。通过左心室球囊尖端导管记录功能参数。再灌注后采集心脏，用氯化三苯四唑染色定量测定梗死面积。另一组心脏（n = 2-3）同样保存，然后研究超微结构和生化变化。结果：0°C保存心脏的组织学和生化分析均未见冻伤迹象。在0°C和4°C条件下，心肌梗死面积无显著差异。在0°C、4°C和8°C储存的心脏中，心肌肌钙蛋白I水平和左心室发展压力具有可比性（均P < 0.05）。结论：0°C保存4小时的心脏与4°C或8°C保存的心脏相比，无冻伤迹象，功能参数相似。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Hypothermic Myocardial Preservation: The Freezing Debate

Background

Storage of hearts in ice for transportation is thought to cause post-transplantation graft dysfunction secondary to freeze injury. We studied the myocardial damage and resulting function in hearts stored at 0 °C, 4 °C and 8 °C for 4 hours.

Methods

Rat hearts were procured under general anesthesia and immediately stored in University of Wisconsin solution at 0 °C, 4 °C and 8 °C for 4 hours (n = 6-8/group). Hearts were then re-perfused on a Langendorff apparatus for 90 minutes using oxygenated Kreb-Henseleit buffer (37 °C). Functional parameters were recorded via a balloon tip catheter in the left ventricle. Following reperfusion hearts were collected for infarct size quantification using triphenyltetrazolium chloride staining. A separate group of hearts (n = 2-3) were similarly stored then studied for ultrastructural and biochemical changes.

Results

There were no signs of freeze injury in hearts stored at 0 °C by histologic or biochemical analysis. There was no significant difference in infarct size between hearts stored at 0 °C and 4 °C. Cardiac troponin I level, and left ventricle developed pressure in hearts stored at 0 °C, 4 °C, and 8 °C were comparable (all P > .05).

Conclusion

Hearts stored at 0 °C for 4 hours showed no signs of freeze injury and had similar functional parameters compared to hearts stored at 4 °C or 8 °C.

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来源期刊

Transplantation proceedings 医学-免疫学

CiteScore

1.70

自引率

0.00%

发文量

502

审稿时长

60 days

期刊介绍： Transplantation Proceedings publishes several different categories of manuscripts, all of which undergo extensive peer review by recognized authorities in the field prior to their acceptance for publication. The first type of manuscripts consists of sets of papers providing an in-depth expression of the current state of the art in various rapidly developing components of world transplantation biology and medicine. These manuscripts emanate from congresses of the affiliated transplantation societies, from Symposia sponsored by the Societies, as well as special Conferences and Workshops covering related topics. Transplantation Proceedings also publishes several special sections including publication of Clinical Transplantation Proceedings, being rapid original contributions of preclinical and clinical experiences. These manuscripts undergo review by members of the Editorial Board. Original basic or clinical science articles, clinical trials and case studies can be submitted to the journal?s open access companion title Transplantation Reports.