小型试验评估食蟹疟原虫和诺氏疟原虫作为间日疟原虫疫苗优先目标的模型。

IF 5 2区医学 Q2 IMMUNOLOGY

Journal of Infectious Diseases Pub Date : 2025-03-14 DOI:10.1093/infdis/jiaf136

Sheena Dass, Prasun Kundu, Deboki Naskar, Usheer Kanjee, Marcelo U Ferreira, Julian C Rayner, Manoj T Duraisingh

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引用次数: 0

摘要

由于缺乏可靠的体外培养系统，间日疟原虫疫苗的发现落后于恶性疟原虫。我们开发了一种微型384孔实验，用于检测进化相关的寄生虫种P. cynomolgi和P. knowlesi，并将其用于筛选针对8种间日疟原虫蛋白的抗体的侵袭抑制效果。所有8种抗体在诺氏疟原虫和食蟹假疟原虫中均表现出一致的抑制作用，这与在离体间日疟原虫分离株中的抑制作用相关。使用类人猿疟疾寄生虫和该试验代表了一种确定间日疟原虫血期候选疫苗优先级的强大、高通量方法。

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Miniaturized assay to evaluate Plasmodium cynomolgi and P. knowlesi as models for prioritizing P. vivax vaccine targets.

Plasmodium vivax vaccine discovery lags behind P. falciparum due to the absence of a reliable in vitro culture system. We developed a miniature 384-well assay for the evolutionarily related parasite species P. cynomolgi and P. knowlesi, deploying it for screening invasion inhibition efficacy of antibodies elicited against 8 P. vivax proteins. All 8 antibodies showed consistent inhibition across both P. knowlesi and P. cynomolgi species, correlating with inhibition in ex vivo P. vivax isolates. The use of simian malaria parasites and this assay represents a robust, high-throughput method for prioritizing P. vivax blood stage vaccine candidates.

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来源期刊

Journal of Infectious Diseases 医学-传染病学

CiteScore

13.50

自引率

3.10%

发文量

449

审稿时长

2-4 weeks

期刊介绍： Published continuously since 1904, The Journal of Infectious Diseases (JID) is the premier global journal for original research on infectious diseases. The editors welcome Major Articles and Brief Reports describing research results on microbiology, immunology, epidemiology, and related disciplines, on the pathogenesis, diagnosis, and treatment of infectious diseases; on the microbes that cause them; and on disorders of host immune responses. JID is an official publication of the Infectious Diseases Society of America.