用彗星法测定尼罗罗非鱼(Oreochromis niloticus)红细胞评估越南河内地区人源化水体的遗传毒性。

IF 2.7 4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES
Elodie Pepey, Gwenn Pulliat, Truong Dinh Hoai, Michaël Bruckert, Geneviève Conéjéro, David Boggio, Coline Perrin, Mathilde Valette, Simon Pouil
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引用次数: 0

摘要

越南河内附近的黑河和Nhue-Day河子流域是重要的水生生态系统,受到工业、家庭和农业来源的严重污染,对环境和公众健康构成威胁。我们在河内及其周边地区沿城市化梯度的四个地点评估了水的遗传毒性:和平水库的一个养鱼场(HB),富宣延区(PX)的一个城郊养鱼场,以及城市湖泊Truc Bach (TB)和Thien Quang (TQ)。利用尼罗罗非鱼红细胞的彗星分析,DNA损伤(%尾DNA),反映了电泳过程中迁移出细胞核的DNA片段,显示了位点之间的显著差异(p
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genotoxic Potential of Anthropized Water Bodies in the Hanoi Region of Vietnam Assessed with the Comet Assay on Erythrocytes of Nile Tilapia (Oreochromis niloticus).

The Black and Nhue-Day River sub-basins near Hanoi, Vietnam, are crucial aquatic ecosystems that are suffering from severe pollution stemming from industrial, domestic, and agricultural sources, which pose risks to environmental and public health. We assessed water genotoxicity at four locations along a gradient of urbanization in Hanoi and its peripheral regions: a fish farm at Hoa Binh reservoir (HB), a peri-urban fish farm in Phu Xuyen district (PX), and urban lakes Truc Bach (TB) and Thien Quang (TQ). Using the comet assay on Nile tilapia erythrocytes, DNA damage (% tail DNA), reflecting fragmented DNA that migrates out of the nucleus during electrophoresis, demonstrated significant differences between sites (p < 0.001). Urban lakes exhibited lower damage (TB: 16 ± 10%, TQ: 33 ± 17%), while the highest damage levels were observed in the hydropower reservoir (HB: 70 ± 15%). Trace elements (i.e., As, Cd, Cr, Ni, and Pb) analyzed in water did not exhibit a significant correlation with DNA damage, suggesting the presence of other unexamined contaminants, such as pesticides, that may explain these findings. These genotoxicity results emphasize the need for further research to identify the specific origins of the observed DNA damage, such as potential contributions from agricultural runoff, untreated wastewater, or other unexamined contaminants. Understanding these sources is essential for developing targeted water management practices to mitigate environmental risks and ensure the safety of aquaculture products, particularly in areas like the HB reservoir, where fish farming supports food security.

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来源期刊
CiteScore
5.60
自引率
3.70%
发文量
230
审稿时长
1.7 months
期刊介绍: The Bulletin of Environmental Contamination and Toxicology(BECT) is a peer-reviewed journal that offers rapid review and publication. Accepted submissions will be presented as clear, concise reports of current research for a readership concerned with environmental contamination and toxicology. Scientific quality and clarity are paramount.
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