巴西绿色蜂胶提取物负载聚(Ε-Caprolactone)纳米粒子包被透明质酸:抗真菌活性外阴阴道念珠菌病小鼠模型

IF 3.4 4区 医学 Q2 PHARMACOLOGY & PHARMACY
Aniely Dos Reis Teixeira, Amanda De Vasconcelos Quaresma, Renata Tupinambá Branquinho, Patrícia Capelari De Oliveira, Jorge Andrés García Suárez, Geraldo Célio Brandão, Ana Paula Moreira Barboza, Marcelo Gonzaga De Freitas Araújo, Juliana Teixeira De Magalhães, Sandra Aparecida Lima De Moura, Gisele Rodrigues Da Silva
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引用次数: 0

摘要

巴西绿色蜂胶提取物负载透明质酸(PE-NPsHA)包被的聚(ε-己内酯)纳米颗粒被开发用于治疗外阴阴道念珠菌病(VVC)和对抗日益严重的真菌耐药性问题。采用UHPLC-MS/MS对其化学成分进行分析,发现其含有酚酸、类黄酮、香豆素、醌类等多种生物活性化合物。FTIR和DSC表明,这些化合物被包裹在NPs的聚合物基质中。采用DLS、AFM、包封率(EE)和体外释放度研究对PE-NPsHA进行了表征。它们呈球形,水动力直径为170 nm,多分散性指数为0.1,zeta电位为-28.5 mV, EE为78%。体外释放研究表明,PE在96 h内具有控释和缓释作用。研究了PE- npsha在体外和体内的生物相容性,并对其在小鼠VVC模型中的抗真菌活性进行了研究。PE-NPsHA没有影响HaCaT细胞的活力,也没有显示出体内阴道毒性的迹象。PE-NPsHA具有体内抗真菌作用,可有效消除白色念珠菌感染。PE-NPsHA可以扩大VVC的可用治疗选择,并抵消念珠菌对抗真菌药物的耐药性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Brazilian Green Propolis Extract-Loaded Poly(Ε-Caprolactone) Nanoparticles Coated with Hyaluronic Acid: Antifungal Activity in a Murine Model of Vulvovaginal Candidiasis

Brazilian green propolis extract-loaded poly(ε-caprolactone) nanoparticles coated with hyaluronic acid (PE-NPsHA) were developed as a therapeutic strategy to treat vulvovaginal candidiasis (VVC) and combat the growing issue of fungal resistance. The chemical composition of PE was analyzed using UHPLC-MS/MS, revealing the presence of various bioactive compounds, such as phenolic acids, flavonoids, coumarins, and quinones. These compounds were encapsulated into the polymeric matrix of NPs, as indicated by FTIR and DSC. In addition, PE-NPsHA were characterized by DLS, AFM, encapsulation efficiency (EE), and in vitro release study. They displayed a spherical morphology with a hydrodynamic diameter of 170 nm, a low polydispersity index of 0.1, a zeta potential of -28.5 mV, and an EE of 78%. The in vitro release study indicated a controlled and sustained release of PE over a period of 96 h. The in vitro and in vivo PE-NPsHA biocompatibility were investigated as well as their antifungal activity in a murine model of VVC. PE-NPsHA did not impact the HaCaT cell viability and demonstrated no signs of in vivo vaginal toxicity. PE-NPsHA exhibited in vivo antifungal efficacy, effectively eliminating Candida albicans infection. PE-NPsHA could expand the available treatment options for VVC and counteract Candida resistance to antifungal drugs.

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来源期刊
AAPS PharmSciTech
AAPS PharmSciTech 医学-药学
CiteScore
6.80
自引率
3.00%
发文量
264
审稿时长
2.4 months
期刊介绍: AAPS PharmSciTech is a peer-reviewed, online-only journal committed to serving those pharmaceutical scientists and engineers interested in the research, development, and evaluation of pharmaceutical dosage forms and delivery systems, including drugs derived from biotechnology and the manufacturing science pertaining to the commercialization of such dosage forms. Because of its electronic nature, AAPS PharmSciTech aspires to utilize evolving electronic technology to enable faster and diverse mechanisms of information delivery to its readership. Submission of uninvited expert reviews and research articles are welcomed.
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