IF 9.7 1区 环境科学与生态学 Q1 AGRICULTURAL ENGINEERING
Fan Liu , Yunpeng Wang , Yijie Wang , Jingwen Zhou , Jianghua Li , Jian Chen , Guocheng Du , Xinrui Zhao
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引用次数: 0

摘要

多亚基血红蛋白可用于人工氧载体、人工食品和医疗诊断,其生物合成已成为一个热门研究课题。然而,不同亚基的不平衡表达和血红素供应不足限制了多亚基血红蛋白的有效生产。通过转录和翻译水平的调控实现不同球蛋白的均衡合成,通过解除血红素对 Hem1p 的反馈抑制改善血红素供应,以及增强酵母中关键限速酶的作用,构建了一个强大的高活性血红蛋白表达平台。合成的高活性血红蛋白包括人血红蛋白(174.2 mg/L,61.7% mol血红素/mol Hb)、猪血红蛋白(203.9 mg/L,72.1% mol血红素/mol Hb)和牛血红蛋白(258.2 mg/L,64.9% mol血红素/mol Hb)。因此,所应用的策略可广泛用于生产其他异源多聚蛋白和血红素结合蛋白。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Efficient synthesis of high-active multi-subunit hemoglobins through balanced expression of globins and robust heme-supply in Saccharomyces cerevisiae

Efficient synthesis of high-active multi-subunit hemoglobins through balanced expression of globins and robust heme-supply in Saccharomyces cerevisiae
The biosynthesis of multi-subunit hemoglobins that can be used in artificial oxygen carriers, artificial foods and medical diagnostics has become a hot research issue. However, the imbalanced expression of different subunits and insufficient heme-supply limit the efficient production of multi-subunit hemoglobins. After the equalized synthesis of different globins through the regulation at transcriptional and translational levels, the improvement of heme-supply by relieving the feedback inhibition of heme on Hem1p and the enhancement of key rate-limiting enzymes in yeast, a robust platform for the expression of high-active hemoglobins was constructed. The synthesis of hemoglobins with high-activity were achieved, including human hemoglobin (174.2 mg/L, 61.7 % mol heme/mol Hb), porcine hemoglobin (203.9 mg/L, 72.1 % mol heme/mol Hb) and bovine hemoglobin (258.2 mg/L, 64.9 % mol heme/mol Hb). Therefore, the applied strategies can be broadly used in the production of other heteromultimeric and heme-binding proteins.
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来源期刊
Bioresource Technology
Bioresource Technology 工程技术-能源与燃料
CiteScore
20.80
自引率
19.30%
发文量
2013
审稿时长
12 days
期刊介绍: Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.
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