不规则根噬菌定殖使c24 -甲基/乙基甾醇比升高。

IF 3.8 2区 生物学 Q2 MYCOLOGY
Pierre-Emmanuel Courty, Jérôme Fromentin, Lucy Martine, Célien Durney, Camille Martin Desbouis, Daniel Wipf, Niyazi Acar, Patricia Gerbeau-Pissot
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引用次数: 0

摘要

植物与微生物的相互作用是许多生态系统作用的基础,特别是通过互惠关系增强植物营养,例如影响大部分陆地植物的丛枝菌根共生。这种相互作用的建立诱导了广泛的信号通路,其中脂质,特别是固醇可能起核心作用。然而,它们所支持的功能却鲜为人知。我们对11种模式植物(香蕉、桶三叶草、亚麻、葡萄藤、玉米、豌豆、杨树、马铃薯、水稻、高粱和番茄)进行了研究,以测量甾醇含量并表征非定殖或被丛枝菌根真菌模型Rhizophagus irregularis DAOM197198定殖的根的甾醇成分。我们的研究结果表明,与未定殖的根相比,定殖根的粗提取物中c24 -甲基甾醇的含量有系统的增加。此外,SMT1和SMT2(分别编码产生c24 -甲基和c24 -乙基甾醇的酶)的转录本在定植的植物根系中也有不同的积累。植物间没有发现共同的调控模式。在100多个植物、蕨类、苔藓、藻类和真菌的全基因组中,SMT1和SMT2家族成员的系统发育关系使鉴定出明确的分支成为可能。因此,我们的研究结果强调了一个保守的丛枝菌根共生依赖于被子植物根固醇组成的调节,具有一些植物特异性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The C24-methyl/ethyl sterol ratio is increased by Rhizophagus irregularis colonization.

Plant-microorganism interactions underlie many ecosystem roles, in particular the enhancement of plant nutrition through mutualistic relationships, such as the arbuscular mycorrhizal symbiosis that affects a large proportion of land plants. The establishment of this interaction induces a wide range of signaling pathways in which lipids, and particularly sterols, may play a central role. However, their supported functions are poorly known. We performed a study on eleven model plants (banana, barrelclover, flax, grapevine, maize, pea, poplar, potato, rice, sorghum and tomato) to measure the sterol content and characterize the sterol composition of roots that were either non-colonized or colonized by the arbuscular mycorrhizal fungal model Rhizophagus irregularis DAOM197198. Our results reveal a systematic increase in the content of C24-methyl sterols in crude extracts of colonized roots as compared to non-colonized roots. In addition, the transcripts of SMT1 and SMT2 (which encode enzymes that produce C24-methyl and C24-ethyl sterols, respectively) were differentially accumulated in colonized plant roots. No common regulation pattern was observed among plants. The phylogenetic relationship of members of the SMT1 and SMT2 families in more than 100 fully sequenced genomes of plants, ferns, mosses, algae and fungi has allowed the identification of unambiguous clades. Our results therefore highlight a conserved arbuscular mycorrhizal symbiosis-dependent regulation of the root sterol composition in angiosperms, with some plant specificities.

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来源期刊
Mycorrhiza
Mycorrhiza 生物-真菌学
CiteScore
8.20
自引率
2.60%
发文量
40
审稿时长
6-12 weeks
期刊介绍: Mycorrhiza is an international journal devoted to research into mycorrhizas - the widest symbioses in nature, involving plants and a range of soil fungi world-wide. The scope of Mycorrhiza covers all aspects of research into mycorrhizas, including molecular biology of the plants and fungi, fungal systematics, development and structure of mycorrhizas, and effects on plant physiology, productivity, reproduction and disease resistance. The scope also includes interactions between mycorrhizal fungi and other soil organisms and effects of mycorrhizas on plant biodiversity and ecosystem structure. Mycorrhiza contains original papers, short notes and review articles, along with commentaries and news items. It forms a platform for new concepts and discussions, and is a basis for a truly international forum of mycorrhizologists from all over the world.
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