Pierre-Emmanuel Courty, Jérôme Fromentin, Lucy Martine, Célien Durney, Camille Martin Desbouis, Daniel Wipf, Niyazi Acar, Patricia Gerbeau-Pissot
{"title":"不规则根噬菌定殖使c24 -甲基/乙基甾醇比升高。","authors":"Pierre-Emmanuel Courty, Jérôme Fromentin, Lucy Martine, Célien Durney, Camille Martin Desbouis, Daniel Wipf, Niyazi Acar, Patricia Gerbeau-Pissot","doi":"10.1007/s00572-025-01193-9","DOIUrl":null,"url":null,"abstract":"<p><p>Plant-microorganism interactions underlie many ecosystem roles, in particular the enhancement of plant nutrition through mutualistic relationships, such as the arbuscular mycorrhizal symbiosis that affects a large proportion of land plants. The establishment of this interaction induces a wide range of signaling pathways in which lipids, and particularly sterols, may play a central role. However, their supported functions are poorly known. We performed a study on eleven model plants (banana, barrelclover, flax, grapevine, maize, pea, poplar, potato, rice, sorghum and tomato) to measure the sterol content and characterize the sterol composition of roots that were either non-colonized or colonized by the arbuscular mycorrhizal fungal model Rhizophagus irregularis DAOM197198. Our results reveal a systematic increase in the content of C24-methyl sterols in crude extracts of colonized roots as compared to non-colonized roots. In addition, the transcripts of SMT1 and SMT2 (which encode enzymes that produce C24-methyl and C24-ethyl sterols, respectively) were differentially accumulated in colonized plant roots. No common regulation pattern was observed among plants. The phylogenetic relationship of members of the SMT1 and SMT2 families in more than 100 fully sequenced genomes of plants, ferns, mosses, algae and fungi has allowed the identification of unambiguous clades. Our results therefore highlight a conserved arbuscular mycorrhizal symbiosis-dependent regulation of the root sterol composition in angiosperms, with some plant specificities.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"35 2","pages":"20"},"PeriodicalIF":3.8000,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The C24-methyl/ethyl sterol ratio is increased by Rhizophagus irregularis colonization.\",\"authors\":\"Pierre-Emmanuel Courty, Jérôme Fromentin, Lucy Martine, Célien Durney, Camille Martin Desbouis, Daniel Wipf, Niyazi Acar, Patricia Gerbeau-Pissot\",\"doi\":\"10.1007/s00572-025-01193-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Plant-microorganism interactions underlie many ecosystem roles, in particular the enhancement of plant nutrition through mutualistic relationships, such as the arbuscular mycorrhizal symbiosis that affects a large proportion of land plants. The establishment of this interaction induces a wide range of signaling pathways in which lipids, and particularly sterols, may play a central role. However, their supported functions are poorly known. We performed a study on eleven model plants (banana, barrelclover, flax, grapevine, maize, pea, poplar, potato, rice, sorghum and tomato) to measure the sterol content and characterize the sterol composition of roots that were either non-colonized or colonized by the arbuscular mycorrhizal fungal model Rhizophagus irregularis DAOM197198. Our results reveal a systematic increase in the content of C24-methyl sterols in crude extracts of colonized roots as compared to non-colonized roots. In addition, the transcripts of SMT1 and SMT2 (which encode enzymes that produce C24-methyl and C24-ethyl sterols, respectively) were differentially accumulated in colonized plant roots. No common regulation pattern was observed among plants. The phylogenetic relationship of members of the SMT1 and SMT2 families in more than 100 fully sequenced genomes of plants, ferns, mosses, algae and fungi has allowed the identification of unambiguous clades. Our results therefore highlight a conserved arbuscular mycorrhizal symbiosis-dependent regulation of the root sterol composition in angiosperms, with some plant specificities.</p>\",\"PeriodicalId\":18965,\"journal\":{\"name\":\"Mycorrhiza\",\"volume\":\"35 2\",\"pages\":\"20\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2025-03-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mycorrhiza\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s00572-025-01193-9\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MYCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycorrhiza","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00572-025-01193-9","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MYCOLOGY","Score":null,"Total":0}
The C24-methyl/ethyl sterol ratio is increased by Rhizophagus irregularis colonization.
Plant-microorganism interactions underlie many ecosystem roles, in particular the enhancement of plant nutrition through mutualistic relationships, such as the arbuscular mycorrhizal symbiosis that affects a large proportion of land plants. The establishment of this interaction induces a wide range of signaling pathways in which lipids, and particularly sterols, may play a central role. However, their supported functions are poorly known. We performed a study on eleven model plants (banana, barrelclover, flax, grapevine, maize, pea, poplar, potato, rice, sorghum and tomato) to measure the sterol content and characterize the sterol composition of roots that were either non-colonized or colonized by the arbuscular mycorrhizal fungal model Rhizophagus irregularis DAOM197198. Our results reveal a systematic increase in the content of C24-methyl sterols in crude extracts of colonized roots as compared to non-colonized roots. In addition, the transcripts of SMT1 and SMT2 (which encode enzymes that produce C24-methyl and C24-ethyl sterols, respectively) were differentially accumulated in colonized plant roots. No common regulation pattern was observed among plants. The phylogenetic relationship of members of the SMT1 and SMT2 families in more than 100 fully sequenced genomes of plants, ferns, mosses, algae and fungi has allowed the identification of unambiguous clades. Our results therefore highlight a conserved arbuscular mycorrhizal symbiosis-dependent regulation of the root sterol composition in angiosperms, with some plant specificities.
期刊介绍:
Mycorrhiza is an international journal devoted to research into mycorrhizas - the widest symbioses in nature, involving plants and a range of soil fungi world-wide. The scope of Mycorrhiza covers all aspects of research into mycorrhizas, including molecular biology of the plants and fungi, fungal systematics, development and structure of mycorrhizas, and effects on plant physiology, productivity, reproduction and disease resistance. The scope also includes interactions between mycorrhizal fungi and other soil organisms and effects of mycorrhizas on plant biodiversity and ecosystem structure.
Mycorrhiza contains original papers, short notes and review articles, along with commentaries and news items. It forms a platform for new concepts and discussions, and is a basis for a truly international forum of mycorrhizologists from all over the world.