液固相萃取-非手性-手性高效液相色谱法测定个人护理用品中泛醇的含量。

IF 2.5 4区 医学 Q2 DERMATOLOGY
Andrea Špačková, Barbora Šimková, Katarína Hroboňová
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引用次数: 0

摘要

目的:建立身体乳、乳霜和乳油的分析方法。目的是建立一种可靠、灵敏和可重复的方法,适用于评估个人护理产品中葡聚糖醇的含量,并确认所申报的对映体形式。方法:对个人护理用品中泛醇的提取工艺进行优化,采用非手性和手性高效液相色谱法(HPLC)结合分光光度法对泛醇及其对映体进行检测和定量。手性高效液相色谱的极化和圆二色检测也被用于确认泛醇的对映体形式。结果:确定了最佳萃取介质为氯仿:水1:3 (v:v)液相萃取,NH2型吸附剂固相萃取。联合提取方法适用于复杂脂肪化妆品样品,体乳、乳霜和油的回收率分别为83.1%、88.2%和83.0% (RSD < 1.6%)。采用以C18型固定相和乙腈:水(10:90,v:v)为流动相的非手性高效液相色谱法和以直链淀粉三(3,5-二甲基苯基氨基甲酸酯)固定相和以正己烷-乙醇(60:40,v:v)为流动相的手性高效液相色谱法分析提取物。结论:所建立的提取方法适用于反相和正相液相色谱系统,适用于多种个人护理用品的分析。在测试样品中检测到d-对映体形式(葡聚糖醇)。体乳、乳霜、油样品中葡聚糖醇的含量分别为0.2% ~ 1.5%、0.04% ~ 0.7%、0.01% ~ 0.03%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Combination of liquid- and solid-phase extraction followed by achiral and chiral high-performance liquid chromatography as an efficient and sensitive method for determination of panthenol in personal care products

Combination of liquid- and solid-phase extraction followed by achiral and chiral high-performance liquid chromatography as an efficient and sensitive method for determination of panthenol in personal care products

Objective

This work deals with the development of an analytical method for the analysis of body milk, cream, and oil. The objective is to develop a reliable, sensitive, and reproducible method suitable to assess the content of dexpanthenol in personal care products and to confirm the declared enantiomeric form.

Methods

Optimization of conditions for the extraction of panthenol from personal care products was carried out for the detection and quantification of panthenol and its enantiomers by achiral and chiral high-performance liquid chromatography (HPLC) with spectrophotometric detection. Polarimetric and circular dichroism detection in chiral HPLC were also used for the confirmation of enantiomeric forms of panthenol.

Results

Optimal extraction media, chloroform:water 1:3 (v:v) for liquid-phase extraction and NH2 type adsorbent for solid-phase extraction have been selected. A combined extraction procedure was useful for the treatment of complex fat cosmetic samples, achieving recoveries of 83.1%, 88.2%, and 83.0% for body milk, cream, and oil, respectively (RSD < 1.6%). The achiral HPLC method with a C18 type stationary phase and acetonitrile:water (10:90, v:v) as a mobile phase, and the chiral HPLC method with an amylose tris(3,5-dimethylphenylcarbamate) stationary phase and a mobile phase composed of n-hexane and ethanol (60:40, v:v) were used for the analysis of extracts.

Conclusion

The proposed extraction procedure is compatible with both the reversed-phase and normal-phase liquid chromatographic systems and is applicable in the analysis of a wide group of personal care products. The d-enantiomeric form (dexpanthenol) was detected in tested samples. The content of dexpanthenol in body milk, cream, and oil samples was found to be 0.2%–1.5%, 0.04%–0.7%, and 0.01%–0.03%, respectively.

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来源期刊
CiteScore
4.60
自引率
4.30%
发文量
73
期刊介绍: The Journal publishes original refereed papers, review papers and correspondence in the fields of cosmetic research. It is read by practising cosmetic scientists and dermatologists, as well as specialists in more diverse disciplines that are developing new products which contact the skin, hair, nails or mucous membranes. The aim of the Journal is to present current scientific research, both pure and applied, in: cosmetics, toiletries, perfumery and allied fields. Areas that are of particular interest include: studies in skin physiology and interactions with cosmetic ingredients, innovation in claim substantiation methods (in silico, in vitro, ex vivo, in vivo), human and in vitro safety testing of cosmetic ingredients and products, physical chemistry and technology of emulsion and dispersed systems, theory and application of surfactants, new developments in olfactive research, aerosol technology and selected aspects of analytical chemistry.
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