黑树莓籽油-化学成分，抗氧化/抗菌活性和体外伤口愈合潜力。

IF 2.5 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia Pub Date : 2025-03-10 DOI:10.1016/j.fitote.2025.106477

Nemanja Krgović , Dejan Stojković , Marija Ivanov , Milena Dimitrijević , Nebojša Menković , Milan Ilić , Miloš S. Jovanović , Katarina Šavikin , Jelena Živković

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引用次数: 0

摘要

本研究旨在研究塞尔维亚黑覆盆子籽油（Rubus occidentalis L.，蔷薇科）的脂肪酸和工具组成、总类胡萝卜素含量的化学特性，并研究其抗氧化/抗菌活性和体外创面愈合潜力。GC/MS分析表明，亚油酸（39.30 %）、α-亚麻酸（30.49 %）和油酸（18.94 %）是主要脂肪酸。HPLC分析显示，γ-生育酚是主要的生育酚异构体（166.80 mg/100 g）。分光光度法测定总类胡萝卜素含量为1.20 mg/100 g。显著的抗自由基活性(DPPH - IC50 3.02 mg/mL；ABTS - IC50 1.33 mg/mL)和较高的还原能力（FRAP值为393.74 μmol Fe2+/100 g）。对鼠伤寒沙门氏菌、大肠杆菌和蜡样芽孢杆菌有显著的抑菌活性，对曲霉属和木霉属菌株也有明显的抑菌活性。细胞活力测定对人角质形成细胞（HaCaT细胞）无细胞毒性作用（IC50 > 401 μg/mL）。用划痕法评价，100 μg/mL黑树莓籽油处理的HaCaT细胞的创面愈合活性（41.77 %）比未处理的细胞（17.34 %）高2.41倍。总的来说，黑覆盆子籽油具有很好的健康潜力。

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Black raspberry seed oil – Chemical composition, antioxidant/antimicrobial activities and in vitro wound-healing potential

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Black raspberry seed oil – Chemical composition, antioxidant/antimicrobial activities and in vitro wound-healing potential

This study aimed to perform chemical characterization of black raspberry seed oil (Rubus occidentalis L., Rosaceae) from Serbia in terms of fatty acids and tocols composition, total carotenoid content, as well as to investigate its antioxidant/antimicrobial activities and in vitro wound-healing potential. GC/MS analysis revealed that linoleic (39.30 %), α-linolenic (30.49 %) and oleic (18.94 %) acid were dominant fatty acids. HPLC analysis highlighted γ-tocopherol as the prevailing tocopherol isomer (166.80 mg/100 g). Spectrophotometric method determined a total carotenoid content of 1.20 mg/100 g. Appreciable antiradical activity (DPPH – IC₅₀ 3.02 mg/mL; ABTS – IC₅₀ 1.33 mg/mL) and a high level of reducing ability (FRAP value of 393.74 μmol Fe²⁺/100 g) were observed. Significant antibacterial activity against Salmonella Typhimurium, Escherichia coli and Bacillus cereus, in addition to antifungal activity against strains from Aspergillus and Trichoderma genera, was demonstrated. By cell viability assay, no cytotoxicity (IC₅₀ > 401 μg/mL) was established on human keratinocytes (HaCaT cells). The wound-healing activity, evaluated by scratch assay, was found to be 2.41-fold higher in HaCaT cells treated with 100 μg/mL of black raspberry seed oil (41.77 %) than in non-treated cells (17.34 %). Taken together, black raspberry seed oil holds promising health potential.

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来源期刊

Fitoterapia 医学-药学

CiteScore

5.80

自引率

2.90%

发文量

198

审稿时长

1.5 months

期刊介绍： Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.