男性皮质星形胶质细胞转录的时间谱预测从早期发育到衰老的分子转变。

IF 5.1 2区 医学 Q1 NEUROSCIENCES
Glia Pub Date : 2025-03-13 DOI:10.1002/glia.70010
Xiaoran Wei, Jiangtao Li, Michelle L. Olsen
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引用次数: 0

摘要

星形胶质细胞是中枢神经系统中最丰富的胶质细胞类型。星形胶质细胞出生在啮齿类动物大脑的产后早期,并与神经元一起成熟,在出生后第二个月表现出显著的形态结构复杂性。在整个发育时期和整个生命周期中,星形胶质细胞参与中枢神经系统的内稳态,支持神经元伙伴,并参与中枢神经系统功能的几乎所有方面。在本研究中,我们分析了野生型雄性啮齿动物一生(出生后7天至18个月)皮质中星形胶质细胞的基因表达。通过对早期发育和中枢神经系统成熟(7-60天)期间差异基因表达的两两时间点比较,发现了四个独特的星形胶质细胞基因簇,每个基因簇都有数百个基因,它们具有独特的时间谱。这些细胞簇与细胞分裂、细胞形态、细胞通讯、维管结构和调控有明显的关系。在成年期和衰老的大脑(3至18个月)中进行的类似分析发现,与细胞代谢和细胞结构相关的分组基因表达模式相似。此外,我们的分析发现,在衰老过程中,星形胶质细胞表现出对较短转录本的偏爱,与突触发育相关的较长基因丢失,与免疫调节和DNA损伤反应相关的较短转录本显著增加。我们的研究强调了星形胶质细胞在整个生命过程中维持中枢神经系统功能的关键作用,并揭示了雄性小鼠皮层在发育和衰老过程中发生的分子变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Temporal Profiling of Male Cortical Astrocyte Transcription Predicts Molecular Shifts From Early Development to Aging

Temporal Profiling of Male Cortical Astrocyte Transcription Predicts Molecular Shifts From Early Development to Aging

Astrocytes are the most abundant glial cell type in the central nervous system (CNS). Astrocytes are born during the early postnatal period in the rodent brain and mature alongside neurons, demonstrating remarkable morphological structural complexity, which is attained in the second postnatal month. Throughout this period of development and across the remainder of the lifespan, astrocytes participate in CNS homeostasis, support neuronal partners, and contribute to nearly all aspects of CNS function. In the present study, we analyzed astrocyte gene expression in the cortex of wild-type male rodents throughout their lifespan (postnatal 7 days to 18 months). A pairwise timepoint comparison of differential gene expression during early development and CNS maturation (7–60 days) revealed four unique astrocyte gene clusters, each with hundreds of genes, which demonstrate unique temporal profiles. These clusters are distinctively related to cell division, cell morphology, cellular communication, and vascular structure and regulation. A similar analysis across adulthood and in the aging brain (3 to 18 months) identified similar patterns of grouped gene expression related to cell metabolism and cell structure. Additionally, our analysis identified that during the aging process astrocytes demonstrate a bias toward shorter transcripts, with loss of longer genes related to synapse development and a significant increase in shorter transcripts related to immune regulation and the response to DNA damage. Our study highlights the critical role that astrocytes play in maintaining CNS function throughout life and reveals molecular shifts that occur during development and aging in the cortex of male mice.

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来源期刊
Glia
Glia 医学-神经科学
CiteScore
13.10
自引率
4.80%
发文量
162
审稿时长
3-8 weeks
期刊介绍: GLIA is a peer-reviewed journal, which publishes articles dealing with all aspects of glial structure and function. This includes all aspects of glial cell biology in health and disease.
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