咖啡因、香兰素及其组合能调节氯化铝诱导的大鼠神经毒性中嘌呤酶活性、某些突触入口蛋白的 mRNA 表达以及海马组织的组织形态学状态

Olakunle Bamikole Afolabi , Kikelomo Folake Jaiyesimi , Oluwaseun Ruth Olasehinde , Oyindamola Adeniyi Olaoye , Lisa Ilobekemen Ekakitie , Adedeji Enitan Adetunji , Adedamola Adediran Fafure , Emmanuel Babatunde Oluwafemi , Omotade Ibidun Oloyede
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引用次数: 0

摘要

铝(Al)的神经毒性与阿尔茨海默病(AD)的发展有关。因此,人们对探索功能性食品中的生物活性化合物如何减轻这种氧化还原金属对AD的有害影响越来越感兴趣。因此,本研究评估了咖啡因、香兰素及其联合使用对alcl3神经毒性大鼠海马部分生化参数的神经调节作用。方法36只成年雄性Wistar大鼠(150 ~ 200 g)随机分为6个治疗组,每组6只。动物口服单剂量AlCl3(100 mg/kg体重,bw)诱导神经毒性。实验用alcl3诱导动物分别给予50 mg/kg bw的咖啡因、香兰素及其联合用药21 d,多奈哌齐(10 mg/kg bw)作为对照。结果经50 mg/kg咖啡因和香兰素联合治疗21天后,海马entpase活性显著降低(p <; 0.05),同时entpase活性显著升高(p <; 0.05)。与未处理alcl3诱导的对照组相比,部分治疗组海马eNOS、AChE和β-淀粉样蛋白相对mRNA基因水平显著(p <; 0.05)降低,GABA受体蛋白表达无显著差异。同样,海马结构显示,治疗21天后,齿状回恢复,颗粒细胞计数增加。结论基于本研究结果,咖啡因、香兰素及其联用可作为治疗AD的潜在候选药物进行进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Caffeine, vanillin and their combination modulate purinergic enzyme activities, mRNA expressions of some synapticsentry proteins and histomorphological status of hippocampal tissue in aluminum chloride-induced neurotoxicity in rats

Background

The neurotoxic properties of aluminum (Al) is associated with Alzheimer's disease (AD) development. Consequently, there has been an increasing interest in exploring how bioactive compounds from functional foods mitigate the detrimental impact of this redox metal in AD. Hence, this study evaluated neuromodulatory effects of caffeine, vanillin and their combination on some hippocampal biochemical parameters in AlCl3-induced neurotoxicity in rats.

Methods

Thirty-six (36) adult male Wistar rats (150–200 g) were randomly divided into 6 treatment groups of 6 rats each. Animals were exposed to AlCl3 (100 mg/kg body weight, bw) orally at a single dose to induce neurotoxicity. Experimental AlCl3-induced animals were administered with 50 mg/kg bw caffeine, vanillin and their combination for 21 days with donepezil (10 mg/kg bw) as control.

Results

However, following the 21 days treatment with 50 mg/kg caffeine, vanillin and their combination, a noticeable significant (p < 0.05) decrease was observed in the hippocampal ENTPDase activity with a concomitant increase significantly (p < 0.05) in eNTDase activity, respectively. Also, a significant (p < 0.05) reduction was evident in the hippocampal relative mRNA gene levels of eNOS, AChE and β-amyloid protein with no significant difference in the expression of GABA receptor protein in some treatment groups compared to untreated AlCl3-induced control group. Similarly, hippocampal architecture revealed a restoration of dentate gyrus with an increased granular cell counts after the 21 days of treatment.

Conclusion

Therefore, based on the findings of this report, caffeine, vanillin and their combination could be further studied as potential candidates for therapeutic management of AD.
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