局部和血清CircHIPK3表达与肝纤维化/肝硬化进展的相关性

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology Pub Date : 2025-02-01 DOI:10.1016/j.ajg.2024.11.003

Ji-Ping Hou , Lian-Ying Yang , Li-Bin Liu , En-Kun Han , Chun-Qi Han , Li-Ping Yang

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引用次数: 0

摘要

背景与研究目的本研究旨在验证血清和组织中环状RNA HIPK3 （CircHIPK3）表达与肝纤维化（LF）和肝硬化（LC）进展的相关性。患者和方法检测120例LF/LC患者和120例健康对照（hc）血清中CircHIPK3的表达。在120个纤维化肝组织中检测到CircHIPK3在组织中的表达，并与57个肝血管瘤患者的健康肝组织进行比较。采用qRT-PCR检测CircHIPK3、TGF-β1、CollA1 mrna的表达。采用Child-Pugh （CP）分级评价疾病严重程度。采用Ishak评分评估肝活检样本的LF/LC。同时测定血清丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）水平。采用受试者工作特征（ROC）分析，评估血清和组织中CircHIPK3表达的诊断价值。结果与hcc相比，LF/LC患者血清和组织中scirchipk3表达上调。A级39例（CP-A）， B级45例（CP-B）， B级36例（CP- c）。与CP-B和CP-A患者相比，CP-C患者血清和局部CircHIPK3水平明显升高。与CP-A相比，CP-B患者血清和组织中CircHIPK3表达上调，差异有统计学意义。ROC曲线分析提示CircHIPK3在血清和组织中的表达可作为LF/LC进展的潜在诊断指标。此外，血清CircHIPK3表达与血清ALT和AST水平呈正相关。组织CircHIPK3表达与组织TGF-β1、CollA1 mRNA表达呈正相关。此外，血清和组织CircHIPK3表达与Ishak评分呈正相关。结论本研究首次证实了CircHIPK3在血清和组织中的表达与LF/LC的进展呈正相关，与病因无关。CircHIPK3可能在LF/LC的发展中发挥重要作用，并作为这些疾病的潜在治疗靶点。

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Correlation of local and serum CircHIPK3 expressions with the progression of liver fibrosis/cirrhosis

Background and study aims

This study was aimed to validate the correlation of circular RNA HIPK3 (CircHIPK3) expression in serum and tissues with the progression of liver fibrosis (LF) and liver cirrhosis (LC).

Patients and methods

Serum CircHIPK3 expressions were detected in 120 patients with LF/LC and 120 healthy controls (HCs). CircHIPK3 expression in tissues was detected in 120 fibrotic liver tissues and compared to 57 healthy liver tissues from patients with hepatic hemangioma. The expressions of CircHIPK3, TGF-β1, and CollA1 mRNAs were assessed by qRT-PCR. The Child-Pugh (CP) classification was used to evaluate disease severity. The Ishak score was applied to assess LF/LC in liver biopsy samples. The levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were also investigated. Receiver operating characteristic (ROC) analysis was conducted to assess the diagnostic value of CircHIPK3 expressions in serum and tissues.

Results

CircHIPK3 expressions in serum and tissues were upregulated in patients with LF/LC compared to HCs. The patient group comprised 39 with CP class A (CP-A), 45 with CP class B (CP-B), and 36 with CP class B (CP-C). Patients with CP-C had markedly increased serum and local CircHIPK3 levels compared to those with CP-B and CP-A. Patients with CP-B showed upregulated CircHIPK3 expressions in serum and tissues compared to CP-A with statistical significance. ROC curve analysis indicated that CircHIPK3 expressions in both serum and tissues may serve as potential diagnostic indicators for the progression of LF/LC. Moreover, serum CircHIPK3 expressions were positively associated with serum ALT and AST levels. Tissue CircHIPK3 expressions were positively correlated with tissue TGF-β1 and CollA1 mRNA expressions. In addition, both serum and tissue CircHIPK3 expressions were positively associated with the Ishak score.

Conclusions

For the first time, this study demonstrated the positive correlation of CircHIPK3 expressions in both serum and tissues with the progression of LF/LC, regardless of etiology. CircHIPK3 might play a significant role in the development of LF/LC and act as a potential therapeutic target for these conditions.

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来源期刊

Arab Journal of Gastroenterology Medicine-Gastroenterology

CiteScore

2.70

自引率

0.00%

发文量

期刊介绍： Arab Journal of Gastroenterology (AJG) publishes different studies related to the digestive system. It aims to be the foremost scientific peer reviewed journal encompassing diverse studies related to the digestive system and its disorders, and serving the Pan-Arab and wider community working on gastrointestinal disorders.