在健康个体中，21天卧床休息导致软骨稳态生物标志物的变化

Osteoarthritis and cartilage open Pub Date : 2025-02-28 DOI:10.1016/j.ocarto.2025.100597

Maren Dreiner , Elie-Tino Godonou , Annegret Mündermann , Koray Tascilar , Georg Schett , Frank Zaucke , Anna-Maria Liphardt , Anja Niehoff

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引用次数: 0

摘要

目的探讨21天卧床固定（有或没有运动和营养干预）对健康人软骨体内平衡生物标志物血清浓度的影响。12名男性志愿者(年龄34.2±8.3岁；BMI（22.4±1.7 kg/m2）的患者参加了6天的基线数据收集（BDC）， 21天的6°头向下倾斜（HDT）卧床休息(CON) +干预HDT +抗振锻炼（RVE）；2次/周;25分钟)和HDT + RVE +营养(NeX；0.6 g/kg体重/天乳清蛋白和90 mmol KHCO3/天碳酸氢盐的补充)，以及6天的恢复(R)。起始HDT条件随机化（CON-RVE-NeX, RVE-NeX-CON, NeX-CON-RVE）。在HDT之前、期间和之后采集血样。分析血清COMP、MMP-3、MMP-9、YKL-40和抵抗素的浓度。结果时间对所有生物标志物的主效应均显著(p <；0.001)。而COMP(- 36%在HDT5， p <；0.001)和MMP-3(- 36%在HDT21， p <；0.001)在HDT卧床休息期间下降，MMP-9 (HDT5时+ 18%，p <；0.001)和抵抗素(HDT21时+ 13%，p <；0.001)在HDT卧床休息期间升高。有趣的是，在恢复期间，YKL-40水平增加（R1时+ 13%，p = 0.022），而MMP-9水平下降（R6时- 19%，p = 0.035）。我们发现COMP和MMP-3之间存在相关性(rrm = 0.58, p <；0.001)以及MMP-9与抵抗素之间(rrm = 0.58, p <；0.001)。结论运动影响软骨体内平衡生物标志物的血清浓度，表明在运动过程中软骨代谢的变化并没有完全恢复。两种干预措施的效果都微乎其微。

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Immobilization by 21-days of bed rest causes changes in biomarkers of cartilage homeostasis in healthy individuals

Objective

To investigate the effects of 21 days bed rest immobilization (with and without exercise and nutrition intervention) on serum concentrations of cartilage homeostasis biomarkers in healthy individuals.

Design

Twelve male volunteers (age 34.2 ± 8.3 years; BMI 22.4 ± 1.7 kg/m²) participated in 6 days of baseline data collection (BDC), 21 days of 6° head-down-tilt (HDT) bed rest (CON) + interventions HDT + resistive vibration exercise (RVE; 2 times/week; 25 min) and HDT + RVE + nutrition (NeX; 0.6 g/kg body weight/day whey protein and 90 mmol KHCO₃/day bicarbonate supplementation), and 6 days of recovery (R) in a cross-over designed study. The starting HDT condition was randomized (CON-RVE-NeX, RVE-NeX-CON, NeX-CON-RVE). Blood samples were collected before, during and after HDT. Serum concentrations of COMP, MMP-3, MMP-9, YKL-40 and resistin were analyzed.

Results

The main effect of time was significant for all biomarkers tested (p < 0.001). While COMP (−36 % at HDT5, p < 0.001) and MMP-3 (−36 % at HDT21, p < 0.001) decreased during HDT bed rest, MMP-9 (+18 % at HDT5, p < 0.001) and resistin (+13 % at HDT21, p < 0.001) increased during HDT bed rest. Interestingly, during recovery, YKL-40 levels increased (+13 % at R1, p = 0.022), while MMP-9 levels decreased (−19 % at R6, p = 0.035). We identified correlations between COMP and MMP-3 (r_rm = 0.58, p < 0.001) as well as between MMP-9 and resistin (r_rm = 0.58, p < 0.001).

Conclusions

Immobilization affects serum concentrations of cartilage homeostasis biomarkers suggesting changes in cartilage metabolism that do not completely recover during re-ambulation. Both interventions had only minimal effects.

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来源期刊

Osteoarthritis and cartilage open Orthopedics, Sports Medicine and Rehabilitation

CiteScore

3.30

自引率

0.00%

发文量