唾液样本作为移植儿童巨细胞病毒再激活诊断和监测的潜在工具。

IF 0.8 4区医学 Q4 IMMUNOLOGY

Transplantation proceedings Pub Date : 2025-03-10 DOI:10.1016/j.transproceed.2025.02.023

Alejandra Pando-Caciano , Pauline E. Mamaní-Cajachagua , Alejandra A. Ingunza-Tapia , Gerardo J. Sánchez-García , Xiomara L. Caffo-Valentín , Emiliana Rizo-Patrón , Sergio A. Murillo-Vizcarra , Holger Maita-Malpartida

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引用次数: 0

摘要

背景：对接受移植的儿童进行巨细胞病毒（CMV）再激活的定期监测是通过血液样本的分子检测进行的。这突出了探索非侵入性替代方法的必要性，比如唾液。本研究评估了唾液样本在秘鲁利马转诊中心接受移植的儿童中检测和监测巨细胞病毒再激活的有效性。方法：采用定量聚合酶链反应（qPCR）对16例移植患儿的85份唾液和全血配对样本及91份拭子唾液和全血配对样本进行分析。结果：全血阳性率为12.59%，液体阳性率为15.29%，唾液阳性率为12.09%。样本之间的病毒载量没有显著差异，唾液和全血之间也没有显著的相关性或一致性。然而，4例患者的19份唾液样本呈阳性，但各自的全血对呈阴性。一名唾液样本呈阳性，血液样本呈阴性的患者发展为胃肠道巨细胞病毒疾病。结论：唾液可能是检测和监测儿童移植受者巨细胞病毒再激活的一种有前景的诊断工具。进一步的研究应探讨唾液病毒载量对指导先发制人治疗和预测疾病发展的意义。

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Saliva Samples as a Potential Tool for the Diagnosis and Monitoring of Cytomegalovirus Reactivation in Children Undergoing Transplantation

Background

Regular monitoring of cytomegalovirus (CMV) reactivation in children who undergo transplantation is performed by molecular testing on blood samples. This highlights the need to explore noninvasive alternatives, such as saliva. The present study evaluated the usefulness of saliva samples for detecting and monitoring CMV reactivation in children undergoing transplantation at a referral center in Lima, Peru.

Methods

A total of 85 paired samples of liquid saliva and whole blood and 91 paired samples of swab-collected saliva and whole blood from 16 children who underwent transplantation were analyzed by quantitative polymerase chain reaction (qPCR).

Results

The positivity rates for whole blood, liquid, and swab-collected saliva were 12.59%, 15.29%, and 12.09%, respectively. No significant differences were observed in viral load among the samples, and there was no remarkable correlation or agreement between saliva and whole blood. However, 19 samples from 4 patients were positive in their saliva but negative in their respective whole blood pairs. One patient who was positive in both the saliva samples and negative in blood developed gastrointestinal CMV disease.

Conclusions

Saliva may serve as a promising diagnostic tool for detecting and monitoring CMV reactivation in pediatric transplant recipients. Further studies should explore the significance of the saliva viral load to guide pre-emptive therapy and predict disease development.

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来源期刊

Transplantation proceedings 医学-免疫学

CiteScore

1.70

自引率

0.00%

发文量

502

审稿时长

60 days

期刊介绍： Transplantation Proceedings publishes several different categories of manuscripts, all of which undergo extensive peer review by recognized authorities in the field prior to their acceptance for publication. The first type of manuscripts consists of sets of papers providing an in-depth expression of the current state of the art in various rapidly developing components of world transplantation biology and medicine. These manuscripts emanate from congresses of the affiliated transplantation societies, from Symposia sponsored by the Societies, as well as special Conferences and Workshops covering related topics. Transplantation Proceedings also publishes several special sections including publication of Clinical Transplantation Proceedings, being rapid original contributions of preclinical and clinical experiences. These manuscripts undergo review by members of the Editorial Board. Original basic or clinical science articles, clinical trials and case studies can be submitted to the journal?s open access companion title Transplantation Reports.