Can Tuzer, Osman Ozan Yegit, Nese Sonmez, Belkis Ertek, Ugur Demirpek, Semra Demir, Ozden Buyukbaba Boral, Suna Buyukozturk, Aslı Gelincik, Bahauddin Colakoglu
{"title":"根除囊虫3亚型可改善慢性自发性荨麻疹的病程。","authors":"Can Tuzer, Osman Ozan Yegit, Nese Sonmez, Belkis Ertek, Ugur Demirpek, Semra Demir, Ozden Buyukbaba Boral, Suna Buyukozturk, Aslı Gelincik, Bahauddin Colakoglu","doi":"10.1159/000545200","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The role of Blastocystis spp. subtypes (STs) in chronic spontaneous urticaria (CSU) and the impact of the eradication are unclear.</p><p><strong>Objective: </strong>To investigate the role of Blastocystis spp. STs in CSU and the impact of the eradication on disease activity.</p><p><strong>Methods: </strong>Stool samples from 295 CSU patients and 38 healthy controls (HCs) were searched for Blastocystis spp. by direct microscopy. Fifty-seven samples from patients and 12 samples from HCs were analyzed for ST distribution by DNA sequencing. DNA sequencing was only performed in samples detected by Real Time (RT)-polymerase chain reaction (PCR). The patients and HCs with Blastocystis spp. formed groups A and B, respectively. Anti-parasitic agents were used to eradicate the parasite in group A. Urticaria medication scores (MSs), urticaria activity score-7 (UAS7), blood eosinophil and basophil counts, total IgE values were compared between groups having different STs at the beginning and six months after the eradication. The subjects who were not taking any medication for the CSU such as antihistamine, montelukast, anti-IgE treatment or cyclosporin had no active urticarial lesions during a 6-month period were considered to be in remission.</p><p><strong>Results: </strong>The parasite was detected in 45.7% (n=135) of the CSU patients and 34.2% (n=13) of the HCs. Fifty-seven samples (85.1%) in 67 CSU patients and 12 samples (92.3%) in 13 HCs revealed positive RT-PCR and formed groups A and B, respectively. ST3 was more frequent in group A (p<0.001) whereas ST1 was more common in group B (p<0.001). The patients having ST3 in group A formed subgroup A1 and other STs in group A formed subgroup A2. After the parasite eradication, the CSU remission rate was higher (p=0.031) and the control values of UAS7, MSs were lower (p=0.028, p=0.049) in subgroup A1 than A2.</p><p><strong>Conclusion: </strong>Although the presence of Blastocystis spp. ST3 does not worsen CSU, its eradication improves the CSU symptoms.</p>","PeriodicalId":13652,"journal":{"name":"International Archives of Allergy and Immunology","volume":" ","pages":"1-21"},"PeriodicalIF":2.5000,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The eradication of Blastocystis spp. subtype 3 improves the course of chronic spontaneous urticaria.\",\"authors\":\"Can Tuzer, Osman Ozan Yegit, Nese Sonmez, Belkis Ertek, Ugur Demirpek, Semra Demir, Ozden Buyukbaba Boral, Suna Buyukozturk, Aslı Gelincik, Bahauddin Colakoglu\",\"doi\":\"10.1159/000545200\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The role of Blastocystis spp. subtypes (STs) in chronic spontaneous urticaria (CSU) and the impact of the eradication are unclear.</p><p><strong>Objective: </strong>To investigate the role of Blastocystis spp. STs in CSU and the impact of the eradication on disease activity.</p><p><strong>Methods: </strong>Stool samples from 295 CSU patients and 38 healthy controls (HCs) were searched for Blastocystis spp. by direct microscopy. Fifty-seven samples from patients and 12 samples from HCs were analyzed for ST distribution by DNA sequencing. DNA sequencing was only performed in samples detected by Real Time (RT)-polymerase chain reaction (PCR). The patients and HCs with Blastocystis spp. formed groups A and B, respectively. Anti-parasitic agents were used to eradicate the parasite in group A. Urticaria medication scores (MSs), urticaria activity score-7 (UAS7), blood eosinophil and basophil counts, total IgE values were compared between groups having different STs at the beginning and six months after the eradication. The subjects who were not taking any medication for the CSU such as antihistamine, montelukast, anti-IgE treatment or cyclosporin had no active urticarial lesions during a 6-month period were considered to be in remission.</p><p><strong>Results: </strong>The parasite was detected in 45.7% (n=135) of the CSU patients and 34.2% (n=13) of the HCs. Fifty-seven samples (85.1%) in 67 CSU patients and 12 samples (92.3%) in 13 HCs revealed positive RT-PCR and formed groups A and B, respectively. ST3 was more frequent in group A (p<0.001) whereas ST1 was more common in group B (p<0.001). The patients having ST3 in group A formed subgroup A1 and other STs in group A formed subgroup A2. After the parasite eradication, the CSU remission rate was higher (p=0.031) and the control values of UAS7, MSs were lower (p=0.028, p=0.049) in subgroup A1 than A2.</p><p><strong>Conclusion: </strong>Although the presence of Blastocystis spp. ST3 does not worsen CSU, its eradication improves the CSU symptoms.</p>\",\"PeriodicalId\":13652,\"journal\":{\"name\":\"International Archives of Allergy and Immunology\",\"volume\":\" \",\"pages\":\"1-21\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2025-03-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Archives of Allergy and Immunology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1159/000545200\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ALLERGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Archives of Allergy and Immunology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1159/000545200","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ALLERGY","Score":null,"Total":0}
The eradication of Blastocystis spp. subtype 3 improves the course of chronic spontaneous urticaria.
Background: The role of Blastocystis spp. subtypes (STs) in chronic spontaneous urticaria (CSU) and the impact of the eradication are unclear.
Objective: To investigate the role of Blastocystis spp. STs in CSU and the impact of the eradication on disease activity.
Methods: Stool samples from 295 CSU patients and 38 healthy controls (HCs) were searched for Blastocystis spp. by direct microscopy. Fifty-seven samples from patients and 12 samples from HCs were analyzed for ST distribution by DNA sequencing. DNA sequencing was only performed in samples detected by Real Time (RT)-polymerase chain reaction (PCR). The patients and HCs with Blastocystis spp. formed groups A and B, respectively. Anti-parasitic agents were used to eradicate the parasite in group A. Urticaria medication scores (MSs), urticaria activity score-7 (UAS7), blood eosinophil and basophil counts, total IgE values were compared between groups having different STs at the beginning and six months after the eradication. The subjects who were not taking any medication for the CSU such as antihistamine, montelukast, anti-IgE treatment or cyclosporin had no active urticarial lesions during a 6-month period were considered to be in remission.
Results: The parasite was detected in 45.7% (n=135) of the CSU patients and 34.2% (n=13) of the HCs. Fifty-seven samples (85.1%) in 67 CSU patients and 12 samples (92.3%) in 13 HCs revealed positive RT-PCR and formed groups A and B, respectively. ST3 was more frequent in group A (p<0.001) whereas ST1 was more common in group B (p<0.001). The patients having ST3 in group A formed subgroup A1 and other STs in group A formed subgroup A2. After the parasite eradication, the CSU remission rate was higher (p=0.031) and the control values of UAS7, MSs were lower (p=0.028, p=0.049) in subgroup A1 than A2.
Conclusion: Although the presence of Blastocystis spp. ST3 does not worsen CSU, its eradication improves the CSU symptoms.
期刊介绍：
''International Archives of Allergy and Immunology'' provides a forum for basic and clinical research in modern molecular and cellular allergology and immunology. Appearing monthly, the journal publishes original work in the fields of allergy, immunopathology, immunogenetics, immunopharmacology, immunoendocrinology, tumor immunology, mucosal immunity, transplantation and immunology of infectious and connective tissue diseases.
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