基于酶联免疫吸附试验（ELISA）的amp活化蛋白激酶（AMPK）活性测定。

IF 2.8 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

FEBS Open Bio Pub Date : 2025-03-11 DOI:10.1002/2211-5463.70017

Trezze P Nguyen, Shangze Lyu, Yang Liu

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引用次数: 0

摘要

amp活化蛋白激酶（AMPK）是细胞和机体能量稳态的主要调节因子，在调节代谢和其他细胞过程中发挥重要作用。由于AMPK活性的药理调节剂具有治疗多种疾病的潜力，人们已经做出了大量的努力。在体外测量AMPK活性是测试AMPK激活剂和抑制剂的基本步骤。在这里，我们报告了一种基于酶联免疫吸附试验（ELISA）的AMPK活性测定方法，步骤简单，灵敏度高。该分析提供了一个强大的，内部替代传统的放射性方法和其他方法，依赖于专门的试剂或商业试剂盒。

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An enzyme-linked immunosorbent assay (ELISA)-based activity assay for AMP-activated protein kinase (AMPK).

AMP-activated protein kinase (AMPK) is the master regulator of cellular and organismal energy homeostasis, playing an essential role in modulating metabolism and other cellular processes. Substantial efforts have been made to develop pharmacological modulators of AMPK activity due to their therapeutic potential against various diseases. Measuring AMPK activity in vitro is a fundamental step for testing AMPK activators and inhibitors. Here, we report an enzyme-linked immunosorbent assay (ELISA)-based AMPK activity assay with simple steps and high sensitivity. This assay offers a robust, in-house alternative to the traditional radioactive methods and other approaches that rely on specialized reagents or commercial kits.

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来源期刊

FEBS Open Bio BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

5.10

自引率

0.00%

发文量

173

审稿时长

10 weeks

期刊介绍： FEBS Open Bio is an online-only open access journal for the rapid publication of research articles in molecular and cellular life sciences in both health and disease. The journal''s peer review process focuses on the technical soundness of papers, leaving the assessment of their impact and importance to the scientific community. FEBS Open Bio is owned by the Federation of European Biochemical Societies (FEBS), a not-for-profit organization, and is published on behalf of FEBS by FEBS Press and Wiley. Any income from the journal will be used to support scientists through fellowships, courses, travel grants, prizes and other FEBS initiatives.