LINC01355/miR-27b-3p靶向POGZ通过与MAD2L2相互作用延缓甲状腺癌进展。

IF 2.6 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

3 Biotech Pub Date : 2025-04-01 Epub Date: 2025-03-08 DOI:10.1007/s13205-025-04231-7

Jiancan Lu, Xinglu Zhou, Hongling Zhu, Mei Zou, Lianyong Liu, Xiangqi Li, Mingjun Gu

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引用次数: 0

摘要

尽管甲状腺癌（THCA）的发病率很高，但其潜在的分子病理学仍然难以捉摸。自闭症相关蛋白POGZ最近参与了肿瘤发生，这激发了我们探索其在THCA中的相关分子调控网络。应用生物信息学分析临床特征及分子间关系。用Co-IP法检测POGZ与MAD2L2的相互作用。通过序列预测和双荧光素酶报告基因检测验证miR-27b-3p与POGZ/LINC01355之间的靶向关系。通过CCK-8实验、克隆形成实验和Transwell实验评估基因的细胞效应，并通过荷瘤裸鼠模型进一步证实。我们的研究结果表明，POGZ在THCA组织和细胞系中的表达减少，并且POGZ与MAD2L2蛋白之间存在相互作用。POGZ抑制THCA细胞的增殖和运动，这些作用在MAD2L2沉默后被逆转。LINC01355在THCA中低表达，与POGZ呈正相关。通过靶向，miR-27b-3p和LINC01355都被确定为POGZ的调节因子。升高的miR-27b-3p抑制POGZ的表达。LINC01355促进POGZ并抵消miR-27b-3p的抑制作用。此外，miR-27b-3p增加THCA细胞的增殖和运动性，这一作用被LINC01355阻断。在动物水平上，POGZ、LINC01355和MAD2L2均能抑制THCA中肿瘤的生长。总的来说，POGZ通过与MAD2L2蛋白相互作用抑制THCA生长，并且POGZ的调节涉及由linc01355靶向的miR-27b-3p协调的复杂相互作用。通过报道首个在THCA中涉及非编码RNA的以pogz为中心的ceRNA网络，我们的研究为探索pogz相关通路和开发新的癌症治疗策略铺平了道路。补充信息：在线版本提供补充资料，网址为10.1007/s13205-025-04231-7。

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POGZ targeted by LINC01355/miR-27b-3p retards thyroid cancer progression via interplaying with MAD2L2.

Despite the high morbidity of thyroid cancer (THCA), the underlying molecular pathology remains elusive. That autism-associated protein POGZ has recently been involved in tumorigenesis intrigues us exploring its relevant molecular regulatory network in THCA. Clinical characteristics and intermolecular relationships were dissected by bioinformatics. Interaction between POGZ and MAD2L2 was examined by Co-IP assay. Targeting relationships between miR-27b-3p and POGZ/LINC01355 was verified by sequence prediction and dual-luciferase reporter detection. Cellular effects of genes were assessed by CCK-8 assay, clone formation assay, and Transwell assay, and further confirmed by a tumor-bearing nude mice model. Our results demonstrated a decrease in POGZ expression in THCA tissues and cell lines, and an interaction between POGZ and MAD2L2 protein. POGZ inhibited both the proliferation and motility of THCA cells, with these effects being reversed upon MAD2L2 silencing. LINC01355 exhibited low expression level and a positive correlation with POGZ in THCA. Both miR-27b-3p and LINC01355 were identified as regulators of POGZ through targeting. Elevated miR-27b-3p suppressed POGZ expression. LINC01355 promoted POGZ and counteracted the inhibitory effects of miR-27b-3p. Furthermore, miR-27b-3p increased the proliferation and motility of THCA cells, an effect that was blocked by LINC01355. At the animal level, POGZ, LINC01355, and MAD2L2 all attenuated tumor growth in THCA. Collectively, POGZ restrains THCA growth by interacting with MAD2L2 protein, and POGZ modulation involves a complex interplay orchestrated by LINC01355-targeted miR-27b-3p. By reporting the first POGZ-focused ceRNA network involving noncoding RNA in THCA, our study paves the way for exploring POGZ-related pathways and developing new therapeutic strategies in cancer.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04231-7.

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来源期刊

3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

CiteScore

6.00

自引率

0.00%

发文量

314

期刊介绍： 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.