蛋白质组学分析确定了禽流感冠状病毒NSP10的细胞内靶标

IF 2.5 4区 医学 Q3 VIROLOGY
Hao Dong, Xueyan Li, Shengkui Xu, Yuxin Wang, Ting Xia, Peng Li, Wenke Ruan
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引用次数: 0

摘要

禽冠状病毒,也称为传染性支气管炎病毒(IBV),是传染性支气管炎(IB)的病原体。IBV的非结构蛋白(NSPs)对病毒复制和逃避宿主免疫应答至关重要。先天免疫反应是抵御病毒感染的第一道防线。IBV基因组编码15个nsp (NSP2-16)。在这项研究中,我们利用共免疫沉淀(Co-IP)和液相色谱-串联质谱(LC/MS/MS)鉴定了与IBV NSP10相互作用的宿主蛋白。蛋白质组学分析显示,宿主蛋白与NSP10的相互作用参与了诸如定位、运输、代谢、细胞周期调节和抗病毒反应等过程。我们进一步探索了NSP10在这些免疫和细胞调控途径中的作用,并证实了NSP10与宿主蛋白hnRNPA1之间的相互作用。进一步研究表明,hnRNPA1抑制IBV复制。推测hnRNP A1与NSP10的结合干扰了复制复合体的功能,从而抑制了病毒复制。然而,NSP10和hnRNP A1的共同过表达部分恢复了病毒复制,这表明这两种蛋白之间存在复杂的关系。这些发现表明,IBV NSP10在病毒感染和调节宿主细胞过程中起着重要作用,突出了其作为治疗干预靶点的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Proteomic analysis identifies intracellular targets for avian coronavirus NSP10

Proteomic analysis identifies intracellular targets for avian coronavirus NSP10

Avian coronavirus, also known as infectious bronchitis virus (IBV), is the causative agent of infectious bronchitis (IB). The non-structural proteins (NSPs) of IBV are critical for viral replication and for evading the host’s immune response. The innate immune response serves as the first line of defense against viral infections. The IBV genome codes for 15 NSPs (NSP2-16). In this study, we identified host proteins interacting with IBV NSP10 using co-immunoprecipitation (Co-IP) and liquid chromatography-tandem mass spectrometry (LC/MS/MS). Proteomic analysis revealed that interactions of host proteins with NSP10 are involved in processes such as localization, transport, and metabolism, regulation of the cell cycle, and antiviral responses. We further explored the role of NSP10 in these immune and cellular regulation pathways and also confirmed the interaction between NSP10 and the host protein hnRNPA1. Further investigation showed that hnRNPA1 inhibited IBV replication. It is speculated that the binding of hnRNP A1 to NSP10 interferes with the function of the replication complex, thereby inhibiting virus replication. However, co-overexpression of NSP10 and hnRNP A1 partially restored viral replication, suggesting a complex relationship between these two proteins. These findings demonstrate that IBV NSP10 plays a significant role in viral infection and in modulating host cell processes, highlighting its potential as a target for therapeutic interventions.

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来源期刊
Archives of Virology
Archives of Virology 医学-病毒学
CiteScore
5.10
自引率
7.40%
发文量
324
审稿时长
4.5 months
期刊介绍: Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.
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