Guangjing Zhang , Jing Huang , Liangyue Peng , Jinhui Liu , Wenbin Liu , Wen Fu
{"title":"定量蛋白质组学分析揭示了斑马鱼初级卵泡亚型的蛋白表达特征","authors":"Guangjing Zhang , Jing Huang , Liangyue Peng , Jinhui Liu , Wenbin Liu , Wen Fu","doi":"10.1016/j.repbre.2025.03.001","DOIUrl":null,"url":null,"abstract":"<div><div>Follicles are the basic structure and functional unit of ovaries. In the previous study, we classified primary follicles (PFs) of zebrafish into four subtypes, compared the DNA methylation and RNA expression characteristics among different subtypes follicles, and preliminarily analyzed the molecular mechanism of PFs development. However, the protein expression characteristics of the four subtypes of PFs remain poorly understood. In this study, a total of 9635 proteins were identified in the four subtypes PFs of zebrafish by proteomic analysis. The proteins highly expressed in the PF-ii and PF-iii were enriched in Notch, MAPK, and mTOR signaling pathways. By combined transcriptomic and proteomic analysis screened five key candidate molecules involved in PFs developmental regulation. These results provide reference to the molecular mechanisms underlying follicular development in fish.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 2","pages":"Pages 21-26"},"PeriodicalIF":0.0000,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Quantitative proteomic analysis revealed the protein expression characteristics of primary follicle subtypes in zebrafish\",\"authors\":\"Guangjing Zhang , Jing Huang , Liangyue Peng , Jinhui Liu , Wenbin Liu , Wen Fu\",\"doi\":\"10.1016/j.repbre.2025.03.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Follicles are the basic structure and functional unit of ovaries. In the previous study, we classified primary follicles (PFs) of zebrafish into four subtypes, compared the DNA methylation and RNA expression characteristics among different subtypes follicles, and preliminarily analyzed the molecular mechanism of PFs development. However, the protein expression characteristics of the four subtypes of PFs remain poorly understood. In this study, a total of 9635 proteins were identified in the four subtypes PFs of zebrafish by proteomic analysis. The proteins highly expressed in the PF-ii and PF-iii were enriched in Notch, MAPK, and mTOR signaling pathways. By combined transcriptomic and proteomic analysis screened five key candidate molecules involved in PFs developmental regulation. These results provide reference to the molecular mechanisms underlying follicular development in fish.</div></div>\",\"PeriodicalId\":74667,\"journal\":{\"name\":\"Reproduction and breeding\",\"volume\":\"5 2\",\"pages\":\"Pages 21-26\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-03-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Reproduction and breeding\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2667071225000122\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reproduction and breeding","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667071225000122","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Quantitative proteomic analysis revealed the protein expression characteristics of primary follicle subtypes in zebrafish
Follicles are the basic structure and functional unit of ovaries. In the previous study, we classified primary follicles (PFs) of zebrafish into four subtypes, compared the DNA methylation and RNA expression characteristics among different subtypes follicles, and preliminarily analyzed the molecular mechanism of PFs development. However, the protein expression characteristics of the four subtypes of PFs remain poorly understood. In this study, a total of 9635 proteins were identified in the four subtypes PFs of zebrafish by proteomic analysis. The proteins highly expressed in the PF-ii and PF-iii were enriched in Notch, MAPK, and mTOR signaling pathways. By combined transcriptomic and proteomic analysis screened five key candidate molecules involved in PFs developmental regulation. These results provide reference to the molecular mechanisms underlying follicular development in fish.
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