寄生甲壳类动物是埃及一些土池养殖设施养殖海鱼感染耐多药耐药溶藻弧菌的潜在媒介:分子、流行病学和病理学证据

IF 1.5 4区 医学 Q3 PARASITOLOGY
Islam A.K. El-waziry , Alaa Eldin Eissa , Reham M. El-Tarabili , Eman M. Abouelhassan , Hanan A. Ghetas , Eman M. Ismail , Mahmoud A. Elnakeeb , Heba A. Abdel Hady , Hadeer S. Aboelnaga , Amina A. Dessouki
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引用次数: 0

摘要

寄生的甲壳类动物影响着土池养殖设施中养殖的海鱼,它们是令人讨厌的寄生虫,会引发一连串的微生物感染,造成经济损失。本文从分类、分子、流行病学和病理学等方面探讨了一些寄生甲壳类动物在养殖海鱼中细菌感染传播中的作用。从一些私人土池养殖设施中随机采集了400种海鱼,其中包括阔步鱼、拉布鱼、野Sparus aurata和罗非鱼。对收集的鱼进行临床检查,以确定是否存在疾病症状、病变和/或寄生虫。从被检查的海鱼的鳃、皮肤和鳍中鉴定出三种寄生甲壳类动物。等足类分别为Livoneca redmanii、Caligus和Lernanthropus。利用16SrRNA进行分子筛选和序列比对,从鱼类和等足类动物中同时分离到溶藻弧菌,两者相似度为99.76 %。有趣的是,73.3 %的分离细菌表现出多重耐药(MDR),多重抗生素耐药指数(MAR指数)≥0.2。此外,56.7% %(17/30)的回收菌株对4种和5种抗菌药物耐药,MAR指数分别为0.50和0.40。组织病理学检查显示受感染鱼的鳃丝和鳍有明显的损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Parasitic crustaceans as a potential vector of MDR Vibrio alginolyticus infection among farmed marine fish in some earthen-pond based Egyptian aquaculture facilities: Molecular, epidemiological and pathological evidences
Parasitic crustaceans affecting cultured marine fish in earthen-pond aquaculture facilities are nasty parasites that trigger cascades of microbial infections with consequent economic losses. In the current study, the role of some parasitic crustaceans in spread and transmission of bacterial infections among cultured marine fishes was investigated utilizing taxonomical, molecular, epidemiological, and pathological assays. A total of 400 marine fish involving Pomadasys stridens, Dicentrarchus labra, Sparus aurata, and Tilapia zilli have been randomly collected from some private earthen pond-based aquaculture facilities. Collected fishes were clinically examined for possible presence of disease symptoms, lesions and/or parasites. Three parasitic crustaceans were identified from gills, skin, and fins of examined marine fish species. The isopod Livoneca redmanii, Caligus, and Lernanthropus species were fully identified. Vibrio alginolyticus was concurrently isolated from both fish and isopod species with 99.76 % similarity between fish and isopod species upon molecular screening utilizing 16SrRNA with sequence alignment. Interestingly, 73.3 % of the isolated bacteria exhibited multi-drug resistance (MDR) with multiple antibiotic resistance (MAR index), values ≥0.2. Further, 56.7 % (17/30) of recovered isolates were resistant to four and five antimicrobials with MAR indices of 0.50 and 0.40. Histopathological examination has revealed remarkable damage of gill filaments and fins of affected fish.
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来源期刊
Parasitology International
Parasitology International 医学-寄生虫学
CiteScore
4.00
自引率
10.50%
发文量
140
审稿时长
61 days
期刊介绍: Parasitology International provides a medium for rapid, carefully reviewed publications in the field of human and animal parasitology. Original papers, rapid communications, and original case reports from all geographical areas and covering all parasitological disciplines, including structure, immunology, cell biology, biochemistry, molecular biology, and systematics, may be submitted. Reviews on recent developments are invited regularly, but suggestions in this respect are welcome. Letters to the Editor commenting on any aspect of the Journal are also welcome.
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