激活肠道菌群-hif1α轴可有效恢复因不适当给药AiiO-AIO6引起的对维罗尼气单胞菌的耐药性。

IF 3.7 3区医学 Q2 NUTRITION & DIETETICS

Journal of Nutrition Pub Date : 2025-05-01 DOI:10.1016/j.tjnut.2025.03.007

Hongwei Yang , Rui Xia , Tsegay Teame , Delong Meng , Shenghui Li , Tiantian Wang , Qianwen Ding , Yuanyuan Yao , Xiaoqing Xu , Yalin Yang , Chao Ran , Yaqing Zhang , Shengkang Li , Benjamin Niemann , Le Luo Guan , Zhen Zhang , Zhigang Zhou

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引用次数: 0

摘要

背景：在斑马鱼饲料中添加淬火酶AiiO-AIO6 3周，可提高斑马鱼的生长性能和抗病性。然而，当饲养期延长至8周时，斑马鱼对维氏气单胞菌的抗病能力下降。目的：研究斑马鱼长期（8周）饲喂AiiO-AIO6添加饲料导致斑马鱼抗病能力下降的机制，并评估饲料添加剂对恢复这种低抗病能力的效果。方法:将1月龄（成年）斑马鱼分别饲喂基础饲料和基础饲料中添加AiiO-AIO6 (10 U/g) 8周（实验1）。此外，建立斑马鱼幼虫模型（实验2），研究AiiO-AIO6对斑马鱼抗病性的影响机制（实验3）。结果表明：AiiO-AIO6对斑马鱼的生长有促进作用，但对斑马鱼的肠道菌群有紊乱作用，对斑马鱼的肠道菌群有紊乱作用，对斑马鱼的抗病性有降低作用（实验5）。斑马鱼幼鱼模型证实，AiiO-AIO6饲喂2 d可提高斑马鱼的抗病能力，饲喂7 d可通过抑制hif1α降低斑马鱼的抗病能力。使用无菌斑马鱼幼虫模型，我们也证明了aiio - aio6诱导肠道微生物群介导的hif1α抑制。此外，饲喂含有三丁酸甘油酯、β-葡聚糖、甘露聚糖或枯草芽孢杆菌的AiiO-AIO6的斑马鱼激活了肠道微生物群-hif1α轴，从而扭转了AiiO-AIO6引起的低抗性。结论：激活肠道菌群-hif1α轴对改善斑马鱼肠道健康和恢复饲料中不适当添加AiiO-AIO6引起的斑马鱼对维罗氏弧菌的低抗性具有重要作用。

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Activation of Gut Microbiota-HIF1α Axis Effectively Restores Resistance to Aeromonas veronii Caused by Improper Administration of AiiO-AIO6

Background

Feeding adult zebrafish a diet supplemented with quenching enzyme AiiO-AIO6 (AIO6) for 3 wk improved the growth performance and disease resistance. However, when the feeding period was extended to 8 wk, zebrafish’s disease resistance to Aeromonas veronii decreased.

Objectives

We investigated the mechanisms of the reduced disease resistance of zebrafish induced by feeding on an AIO6 supplemented diet for a long term (8 wk) and assessed the effectiveness of feed additives in restoring the low disease resistance.

Methods

One-month-old (adult) zebrafish were fed with a basal diet and the basal diet supplemented with AIO6 (10 U/g) for 8 wk (experiment 1). Furthermore, the zebrafish larvae model (experiment 2) was developed and used to study the mechanisms of how AIO6 affected disease resistance (experiment 3). We also investigated the effectiveness of selected prebiotic tributyrin, β-glucan or mannan in activating gut microbiota- HIF1α to restore the low disease resistance of adult zebrafish fed with AIO6 for 8 wk (experiment 4). Lastly, the effects of Bacillus subtilis in activating the gut microbiota-HIF1α and improving the low disease resistance of zebrafish larvae induced by AIO6 were examined (experiment 5).

Results

Feeding adult zebrafish with AIO6 for 8 wk promoted growth but disordered the gut microbiota and reduced disease resistance. The zebrafish larvae model confirmed that feeding AIO6 for 2 d increased disease resistance, whereas 7 d decreased the resistance by suppressing HIF1α. Using a germ-free zebrafish larvae model, we also demonstrated that AIO6-induced gut microbiota mediated inhibition of HIF1α. Furthermore, zebrafish fed on the AIO6-containing diet supplement with tributyrin, β-glucan, mannan, or Bacillus subtilis activated the gut microbiota-HIF1α axis to reverse the low resistance caused by AIO6.

Conclusions

Activating the gut microbiota-HIF1α axis has a vital role in improving intestinal health and restores the low resistance to Aeromonas veronii caused by improper administration of dietary AIO6 in zebrafish.

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来源期刊

Journal of Nutrition 医学-营养学

CiteScore

7.60

自引率

4.80%

发文量

260

审稿时长

39 days

期刊介绍： The Journal of Nutrition (JN/J Nutr) publishes peer-reviewed original research papers covering all aspects of experimental nutrition in humans and other animal species; special articles such as reviews and biographies of prominent nutrition scientists; and issues, opinions, and commentaries on controversial issues in nutrition. Supplements are frequently published to provide extended discussion of topics of special interest.