Daniel J E McKnight, Johanna Wong-Bajracharya, Efenaide B Okoh, Fridtjof Snijders, Fiona Lidbetter, John Webster, Mathew Haughton, Steven P Djordjevic, Daniel R Bogema, Toni A Chapman
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引用次数: 0
摘要
1976年至2010年期间,在澳大利亚新南威尔士州和昆士兰州收集了四种细菌分离株,并作为常规生物安全监测的一部分进行了储存。最近,作为一个更大项目的一部分,对这些历史分离株进行了分析,以提高我们培养收集的分类准确性,并改善澳大利亚的生物安全准备。从紫堇中分离到3株黄单胞菌,初步鉴定为黄单胞菌属;从紫花苜蓿中分离到1株黄单胞菌,初步鉴定为轴索黄单胞菌亚种。alfalfae。在这项研究中,我们采用现代表型和基因组技术来进一步表征这些分离株。基质辅助激光解吸电离飞行时间MS生物分型和bio GEN III MicroPlates证实它们是黄单胞菌属的成员,但不允许物种水平的分类。基因组关联指数和系统发育分析证实它们是黄单胞菌,并表明它们代表三个新种。4株菌株与所有黄单胞菌型菌株的最大平均核苷酸一致性和数字DNA-DNA杂交值分别为93.9%和50.7%。发病机理分析证实,其中两个分离株对分离它们的植物Fragaria不具有致病性。在此基础上,我们提出了11月黄单胞菌属(DAR 80977T=ICMP 24943)、11月药物黄单胞菌属(DAR 35659T=ICMP 24942)和11月黄单胞菌属(DAR 34887T=ICMP 24940)的命名。
Xanthomonas bundabergensis sp. nov., Xanthomonas medicagonis sp. nov. and Xanthomonas tesorieronis sp. nov.: three members of group 1 Xanthomonas.
Between 1976 and 2010, four bacterial isolates were collected in New South Wales and Queensland, Australia, and stored as part of routine biosecurity surveillance. Recently, these historic isolates were analysed as part of a larger project to enhance the taxonomic accuracy of our culture collection and improve Australia's biosecurity preparedness. Three isolates were collected from Fragaria × ananassa, initially identified as Xanthomonas sp., and one from Medicago sativa, identified as Xanthomonas axonopodis subsp. alfalfae. In this study, we employed modern phenotypic and genomic techniques to further characterize these isolates. Matrix-assisted laser desorption ionization-time of flight MS biotyping and Biolog GEN III MicroPlates confirmed that they are members of the Xanthomonas genus but did not allow for species-level classification. Genome-relatedness indices and phylogenetic analysis confirmed that they were Xanthomonas and revealed that they represent three novel species. The maximum average nucleotide identity and digital DNA-DNA hybridization values observed when comparing the four isolates to all Xanthomonas type strains and each other were 93.9% and 50.7%, respectively. Pathogenesis assays confirmed that two of the isolates are not pathogenic to Fragaria, the plant from which they were isolated. Based on these findings, we propose the names Xanthomonas bundabergensis sp. nov. (DAR 80977T=ICMP 24943), Xanthomonas medicagonis sp. nov. (DAR 35659T=ICMP 24942) and Xanthomonas tesorieronis sp. nov. (DAR 34887T=ICMP 24940).
期刊介绍:
Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names.
The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including:
Identification, characterisation and culture preservation
Microbial evolution and biodiversity
Molecular environmental work with strong taxonomic or evolutionary content
Nomenclature
Taxonomy and phylogenetics.