安非他酮通过降低氧化应激和炎症反应对脑缺血再灌注损伤具有神经保护作用

IF 1.8 4区 医学 Q3 PHARMACOLOGY & PHARMACY
Iranian Journal of Pharmaceutical Research Pub Date : 2025-01-04 eCollection Date: 2024-01-01 DOI:10.5812/ijpr-156838
Saeid Baba Ahmadi, Zeinab Afrand Khalilabad, Seyedeh Sepideh Alemohammad, Hasan Yousefi Manesh, Alireza Abdollahi, Farahnaz Jazayeri, Seyyedeh Elaheh Mousavi
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引用次数: 0

摘要

背景:脑缺血/再灌注(I/R)损伤是脑卒中最常见的形式,影响世界各地的许多患者。据信,氧化应激和炎症在疾病开始后发生的损伤中起主要作用。因此,本研究首次在大鼠模型上探讨安非他酮对脑I/R损伤的神经保护作用。方法:40只雄性大鼠分为正常组、脑I/R组和2个病变组,分别给予60和100 mg/kg安非他酮。诱导疾病一天后,对大鼠进行行为测试,包括网格行走、新物体识别和修正神经严重程度评分(mNSS)。测定大鼠脑匀浆中IL-1β、TNF-α、IL-6、IL-10等炎性细胞因子水平。此外,测定MDA、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、还原性谷胱甘肽(GSH)和NO2 -的水平。结果:安非他酮给药与脑I/R大鼠在新物体识别和网格行走行为测试中的表现改善以及神经障碍评分有关。此外,bcaao诱导的炎症可以通过降低细胞因子IL-1β、TNF-α和IL-6的水平以及上调IL-10来减轻。此外,100 mg/kg安非他酮可降低脑I/R大鼠的膜脂过氧化,提高SOD活性水平。最后,安非他酮可抑制BCAAO诱导的NO2 -水平升高。结论:安非他酮对脑I/R损伤具有神经保护作用,其机制可能与降低脑内炎症和氧化应激有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bupropion Showed Neuroprotective Impacts Against Cerebral Ischemia/Reperfusion Injury by Reducing Oxidative Stress and Inflammation.

Background: Cerebral ischemia/reperfusion (I/R) injury is the most prevalent form of brain stroke, affecting many patients worldwide. It is believed that oxidative stress and inflammation play major roles in the damage that occurs after the initiation of the disease.

Objectives: Therefore, for the first time, the current study aimed to investigate the neuroprotective effects of bupropion against cerebral I/R damage in a rat model.

Methods: Forty male rats were divided into four groups: Control, cerebral I/R, and two diseased groups that received 60 and 100 mg/kg of bupropion. One day after induction of the disease, behavioral tests, including grid walking, novel object recognition, and modified neurological severity score (mNSS), were performed on the rats. The levels of inflammatory cytokines, including IL-1β, TNF-α, IL-6, and IL-10, were measured in the rats' brain homogenates. Additionally, the levels of MDA, catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH), and NO2 - were measured.

Results: Bupropion administration was associated with improved performance in the novel object recognition and grid walking behavioral tests, as well as in the neurological disorder scores, in cerebral I/R rats. Moreover, BCAAO-induced inflammation was reduced by the administration of this drug, evidenced by reduced levels of cytokines IL-1β, TNF-α, and IL-6 and upregulation of IL-10. Additionally, membrane lipid peroxidation was reduced in the cerebral I/R rats receiving 100 mg/kg bupropion, and the level of SOD activity was improved in these animals. Finally, the administration of bupropion prevented the increase in NO2 - levels induced by BCAAO.

Conclusions: In conclusion, bupropion has neuroprotective effects against cerebral I/R damage by reducing inflammation and oxidative stress in the brain.

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来源期刊
CiteScore
3.40
自引率
6.20%
发文量
52
审稿时长
2 months
期刊介绍: The Iranian Journal of Pharmaceutical Research (IJPR) is a peer-reviewed multi-disciplinary pharmaceutical publication, scheduled to appear quarterly and serve as a means for scientific information exchange in the international pharmaceutical forum. Specific scientific topics of interest to the journal include, but are not limited to: pharmaceutics, industrial pharmacy, pharmacognosy, toxicology, medicinal chemistry, novel analytical methods for drug characterization, computational and modeling approaches to drug design, bio-medical experience, clinical investigation, rational drug prescribing, pharmacoeconomics, biotechnology, nanotechnology, biopharmaceutics and physical pharmacy.
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